Method for rapid thermal cycling of biological samples A thermal cycling method and device is disclosed. The device comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such a ... ...
The invention discloses a method for preparing cephalosporium acremonium proteome, which comprises the following steps that: cephalosporium acremonium thalli are ground into powder with liquid nitrogen, and then, total protein samples are extracted by a trichloroacetic acid (TCA)-acetone precipitation ...
and a concentration of protein samples was detected by a BCA kit. SDS-PAGE electrophoresis gel was prepared, protein electrophoresis was implemented, a membrane was transferred by a wet process, and sealing was carried out. Antibody incubation: corresponding to a size of a protein marked by prote...
Once biochemical readiness has been determined the sample optimization for plunge freezing can begin. In the next post we will look at the considerations and methods for optimizing samples for conventional sample vitrification for cryo-EM. > N...
The LPP strain was obtained from fermented sorghum grain. Samples taken from the latter were inoculated into MRS (Merck) broth and incubated at 30° C., without aeration for 2 to 3 days. The culture was streaked out onto MRS Agar (Merck) plates and incubated under the same conditions for...
For the analysis, 50 μg samples of total RNA were tested with the use of northern blots to determine the presence of the desired transcripts. 7. Protein Extraction For the extraction of the total protein from plant tissues, tissue pieces were homogenized in protein extraction buffer (25 mM...
Hanton, SDStets, JRJournal of the American Society for Mass SpectrometryHanton SD, Stets JR (2009) Determining the time needed for the vortex method for preparing solvent-free MALDI samples of low molecular mass polymers. J Am Soc Mass Spectrom 20(6):1115- 1118...
SDS PAGE electrophoresis was performed to assess molecular weight of proteins of the extract. The peach flower extract is heated to 70° C. for 10 minutes in reductive denaturing conditions in a denaturing sample buffer. An antioxidant solution is added to the inner chamber (cathode) so that th...
The purification of the enzyme throughout the process can be followed by SDS-PAGE. FIG. 4B of the drawings is a reproduction of the photograph of the gel following electrophoresis on samples of the enzyme at the various stages of the process. As shown by FIG. 4B, the lane corresponding to...
After adding 200 μΙ of an aqueous solution of lichenase enzyme (10 U) to each test tube the samples were stored in the water bath for 1 h. Sodium acetate buffer (5 ml, 200 mM, pH 4) was added to each tube. The tubes were centrifuged at 1000 rpm for 15 min. One hundred μ...