A single colony ofE. coliDSM14459 (pAM10.1, pNO4) was incubated, at 37° C. for 18 hours and with shaking (250 rpm), in 2 ml of LB medium (10 g of yeast extract/l, 5 g of tryptone/l, 10 g of NaCl/l) in the added presence of antibiotics (50 μg of ampicillin/l and ...
The actual assay is performed by mixing 100 μl of this assay reagent with 100 μl of the lipase sample. The color development is compared to that of a known lipase sample. The brilliant green plates consisted of 15 ml LB agar, with a top layer of 3 ml LB agar containing 0.3 ml ...