If the surfactant is ionic and contains a single hydrocarbon chain (e.g., sodium dodecyl sulfate, SDS) microemulsions are only formed if a cosurfactant (e.g., a medium size aliphatic alcohol) and/or electrolyte (e.g., 0.2 M NaCl) are also present. With double-chain ionic (e.g., ...
(10ul; 20,000 cpm) with or without the test sample and the mixture was incubated at 37° C. for 90 minutes. The mixture was then diluted with ice-cold 50 mM Tris-0.9% NaCl, pH 7.4 (4 ml) and filtered through a glass fiber filter (GF/B Whatman 2.4" diameter). The filter was ...
The assay plate was prepared with 1 mg ml21 HA, 1.5% agarose, 50 mM sodium citrate (pH 5.5) buffer, 0.15 M NaCl and 0.02% (w/v) sodium azide. The fermentation was emptied into the cylindrical holes (3 mm in diameter) on the agarose plates, incubated at 37uC for h and covered ...
150 mM NaCl 8.76 ml 1% Triton X-100 or NP-40 10 ml 0.5% Sodium deoxylcholate 5 g 0.1% SDS 1 g 1 mM EDTA (0.5M stock) 2 ml 10 mM Naf 0.42 g Add ddH2O to 1000 ml Add PMSF to a final concentration of 1mM and any other protease inhibitors immediately before use. Last update...
of deionized water was added and the tube was shaken in a horizontal shaker for 15 min. Next, 25 ml acetonitrile was added and again the tube was shaken in a horizontal shaker for 15 min. 4.0 g of anhydrous MgSO4and 2 g of NaCl were added and again the tube was shaken in a ...
Horse spleen apoferritin was purchased from Sigma Aldrich (A3660), and exchanged into 30 mM HEPES, 150 mM NaCl pH 7.5 by ultrafiltration using 100 kDa molecular weight cut-off (MWCO) spin concentrator tubes (Vivaspin, Sartorius). Protein concentration was then determined using absorbance at 280 ...
The supernatant was added to another extraction tube containing 0.2 g of NaCl and 0.8 g of MgSO4, followed by a 1-min vortex mixing step. After centrifugation (10 min at 1200× g), 500 µL of the supernatant was added to an autosampler vial and diluted by the addition of 200 µL...
Meanwhile, each protein sample (10 mg) was dissolved in 1 mL of phosphate buffered saline (PBS; 10 mmol/L phosphate buffer and 150 mmol/L NaCl; pH 7.0) and added to the mixture. The mixture was left at room temperature for 1.5 h to complete the reaction. The NIV-KLH conjugate was ...
Passing a direct current of electricity through a solution of NaCl causes the chloride ions to migrate to the positive electrode where oxidation to gaseous chlorine occurs when the ion gives up an electron to the electrode: 2Cl−(aq)→Cl2(g)+2e−(at the positive electrode)2C...
CO2 absorption enhancement by Al2O3 nanoparticles in NaCl aqueous solution. Energy 2020, 53, 206–211. [Google Scholar] [CrossRef] Vajjha, R.; Das, D. Experimental determination of thermal conductivity of three nanofluids and development of new correlations. Int. J. Heat Mass Transf. 2009,...