Abstract We provide a protocol for precision nuclear run-on sequencing (PRO-seq) and its variant, PRO-cap, which map the location of active RNA polymerases (PRO-seq) or transcription start sites (TSSs) (PRO-cap) genome-wide at high resolution. The density of RNA polymerases at a particular...
One powerful method, Precision nuclear Run-On sequencing (PRO-seq), maps the locations of RNA polymerase active sites genome-wide at high resolution. PRO-seq provides a snapshot of strand-specific transcriptional activity and does not rely on immunoprecipitation of the polymerase of interest. ...
Additionally, negative nuclear RAD51 staining correlated with lymph node metastasis, larger tumor size, and lower disease-free survival in gastric adenocarcinoma31. These findings indicate that HR genes may serve divergent roles across tumor types. Further research is warranted to better understand this...
adding nuclear-localization sequences (NLS) and preforming codon optimization can ensure the base editor gets well expressed in the nucleus [118,119]. On the other side, co-expressing free UGI [120] or fusing more UGI to CBE components [121] for reducing the frequency of uracil excision. I...
The first major challenge is improving on-target editing efficiency. On one side, adding nuclear-localization sequences (NLS) and preforming codon optimization can ensure the base editor gets well expressed in the nucleus [118,119]. On the other side, co-expressing free UGI [120] or fusing ...
MRI 3D Studying magnetic features of tissues by employing the effect of nuclear magnetic resonance Soft tissues Magnetic features of masses, fluids, cells and tissues Neurology (e.g., ischemic stroke), vertebrology, oncology, muscular-skeletal radiology (joints, tendons, bone marrow), cardiology (...
We thank Her Majesty Queen Elizabeth II for starting the sequencing run containing our samples and Andrew J. Steele for comments on the machine learning analysis. This work was supported by the Francis Crick Institute, which receives its core funding from Cancer Research UK (grants FC001110 and...
The nucleus is surrounded by a membrane called the nuclear envelope, which protects the DNA and separates the nucleus from the rest of the cell. (illustration on page 9) The plasma membrane is the outer lining of the cell. It separates theHandbook Table of Contents...
2a). Tissues were stained with DAPI for nuclear visualization. A crucial step in DVP is delineation of the cell plasma membrane for subsequent laser microdissection (Fig. 2c). For our samples, we found that staining with wheat germ agglutinin (WGA), a lectin that binds to specific ...
To assemble a repertoire of neoantigens, we conducted exome and transcriptome sequencing of subcutaneous CT26 tumours. Neoantigens were predicted from highly expressed tumour-specific mutations using established methods14,15, with selection criteria inclusive of putative neoantigens across a spectrum of MH...