探针PRCC/TFE3融合基因t(X;1)探针试剂(荧光原位杂交法)代码:F.01257-01管理类别:Ⅰ类探针名称:GSP PRCC / GSP TFE3供货周期:一周注册证号:粤穗械备20191046号 临床意义 肾脏肿瘤 Xp11.2 易位型肾细胞癌中发生率最高的两个融合基因分别为ASPSCR1-TFE3和PRCC-TFE3; TFE3融合亚型,更多地表现出巢状或实性...
PRCC/TFE3融合基因检测-分子病理-广州安必平医药科技股份有限公司(股票代码:688393)专注于肿瘤筛查与诊断,自主研发了一系列肿瘤筛查及诊断试剂与配套设备,主要应用科室为病理科。目前已搭建液基细胞学(LBP)、聚合酶链式反应(PCR)、免疫组织化学(IHC)和荧光原位杂
PRCC-TFE3 translocation renal cell carcinomas (tRCC) isa common subtype of TFE3 tRCCs in which TFE3 fusions areindicated as oncogenes to promote tumor development. PRCC-TFE3 fusions are often accumulated in the nucleus and related to poorer outcomes and higher stages (III/IV). In this ...
保证杂交的每一条探针序列的高特异性;同时精确控制目标区域的靶向,获得说明书表1中所示的1533个核苷酸序列,再在每个核苷酸序列的两端添加标签序列得到特征性引物,以特征性引物为模板,PCR扩增,纯化后得到探针组.采用上述探针组用于检测PRCC/TFE3基因融合状态,成本较低且在30分钟左右即可完成骨髓样本的FISH杂交,敏感性高...
PRCC, the commonest TFE3 fusion partner in papillary renal carcinoma is associated with pre-mRNA splicing factors.In papillary renal cell carcinomas the TFE3 transcription factor becomes fused to the PSF and NonO pre-mRNA splicing factors and most commonly to a protein of unknown function ...
近日,中国医学科学院肿瘤医院寿建忠教授团队建立并验证了转移性PRCC-TFE3融合异位性肾细胞癌(tRCC)患者的类器官(Organoids),并首次实现了高通量筛选(High-throughput screening,HTS)过程,希望通过HTS寻找潜在治疗药物,并通过RNA-seq和细胞研究探索其可能的机制。
(1996a). Fusion of the transcription factor TFE3 gene to a novel gene, PRCC, in t(X;1)(p11;q21)- positive papillary renal cell carcinomas. Proc. Natl. Acad. Sci. U.S.A. 93, 15294-15298. doi: 10.1073/pnas.93.26.15294Weterman M.A., Wilbrink M., and Geurts van Kessel A. ...
Xp11 translocation renal cell carcinomas (RCCs) are uncommon renal tumors, character- ized by several different translocations involv- ing the TFE3 gene [1]. In these diseases, the TFE3 gene is fused by translocation to 1 of sev- eral other genes, including ASPL, PRCC, NONO (p54nrb), ...
本发明涉及基因检测技术领域,具体涉及一种检测PRCC/TFE3基因的荧光原位杂交探针组及其制备方法和应用.本发明通过在PRCC和TFE3基因的目标区域,构建与目标区域完全互补的长度为45bp的单链片段作为候选探针,然后将每一条序列进行批量BLAST比对,保证杂交的每一条探针序列的高特异性;同时精确控制目标区域的靶向,获得说明书表...
本发明公开用于检测样本中PRCCTFE3融合基因的寡核苷酸,方法和试剂盒,其包括针对1型PRCCTFE3的上下游引物和探针,针对2型PRCCTFE3的上下游引物和探针,针对3型PRCCTFE3的上下游引物和探针,针对4型PRCCTFE3的上下游引物和探针,以及针对βactin内参基因的上下游引物和探针.本发明可快速检测样本中是否存在PRCCTFE3融合...