Dysfunction of PRC2, but not KMT2D-COMPASS, yields a quasi-mesenchymal state that is associated with highly metastatic capabilities and poor survival of patients with breast cancer, suggesting that great caution should be applied when PRC2 inhibitors are evaluated clinically in certain patient cohorts...
We and others have shown that resident cancer stem cells (CSCs) are significantly enriched in EOC, a population thought to underlie tumor recurrence, chemoresistance, and metastasis [2]. Therapeutics to target CSCs are in development and undergoing evaluation in the clinic, thus warranting additional...
Cells were lysed using RIPA buffer (Pierce) containing protease inhibitors cocktail (Roche) and proteins were extracted according to the manufacturer's instruction. Proteins were separated by electrophoresis in 10% SDS polyacrylamide gels and transferred to polyvinylidene fluoride membranes (Millipore). Pri...
S3B). At the same doses of arginine methyltransferase inhibitors, there was no reduction in H3K4me3 or H3K27me3 on the transgenic H3WT tail or gain of either on the H3R2C mutant histone tail (Supplementary Fig. S3C). On the contrary, co-inhibition of type I and II arginine ...
and cisplatin resistance underlie the poor overall survival. We and others have shown that resident cancer stem cells (CSCs) are significantly enriched in EOC, a population thought to underlie tumor recurrence, chemoresistance, and metastasis [2]. Therapeutics to target CSCs are in development and ...
Fresh 0.5 mM DTT, 0.2 mM PMSF, and 50× protease inhibitors were added and the cells were centrifuged at 2500 × g for 5 min at 4 °C. The supernatant was discarded, and the pellet resuspended in 500 µl Buffer A containing 0.2% NP-40 per 10 million cells and ...
S3B). At the same doses of arginine methyltransferase inhibitors, there was no reduction in H3K4me3 or H3K27me3 on the transgenic H3WT tail or gain of either on the H3R2C mutant histone tail (Supplementary Fig. S3C). On the contrary, co-inhibition of type I and II arginine ...