RNF2 (also known as RING1B), a catalytic subunit of PRC1, was highly expressed in Sertoli cells, as indicated by co-localization with the Sertoli cell marker GATA4, and the RNF2-mediated mark, H2AK119ub, was abundant in Sertoli cells (Fig. 1a, Supplementary Fig. 1), which suggest ...
36,37. To ensure that the function of each PRC2 component was completely eliminated, we designed a targeting strategy to ablate the entire functional domain critical
we reported that chromosome silencing is significantly reduced in the absence of both PRC1 and PRC2 (Almeida et al., 2017). Accordingly, in this study we observed a similar reduction in silencing following deletion of the XR-PID element. We were able to examine this effect in ...
Total RNA was purified by RNeasy Minikit (QIAGEN) according to the manufacturer’s manual. RNA concentration was determined using Nanodrop, and 500 ng of total RNA was used for library construction using the KAPA Stranded mRNA-seq Kit. Sequencing was performed on Illumina HiSeq 2500 machines wit...
According to a 2022 survey by nurse staffing agency ConnectRN, 61% of the nurses who were considering leaving the profession said the top reason is the impact of nursing shortages. Lack of support for entry-level nurses Turnover rates for new nurses are as high as 30% in the first year ...