RNA probes were designed and purchased from ACDBio; Snip1 probe targeting 1270-2274 bp of NM_001356560.1 and Eomes/Tbr2 probe targeting 1289-2370 bp of NM_010136.3 (Cat. 429649-C2). Sectioning and in situ hybridization (ISH) were done by the Comparative Histology Core at St. Jude ...
factors that are lowly expressed in early embryonic cells and are poised for lineage-specific expression during differentiation in mammals. However, the bivalency of the mammalian genes occurs mainly at the promoters or enhancers. In addition, the rice genes marked by H3K4me3 and H3K27me3 are de...
Serial dilutions of PRC2 proteins were made in EBB, and then an equal volume of 25 nM nucleosomes in EBB plus 0.5 mg/ml BSA was added to each PRC2 dilution. Reactions were incubated at room temperature for 30 min, loaded onto 3.5% native polyacrylamide gels, and run at 120...
The data from this example show the surface-immobilized microtubule moving at a constant velocity of 50 nm/s, the free microtubule held in place via the optically trapped bead, and the GFP-PRC1 molecules clustering into the region of decreasing overlap (Figure 1B). Upon complete separation of...
After cells reached an absorbance of 0.8–1.0 at 600 nm (around 4 h at 37 ∘°C), they were added to 500 mL of M9 minimal medium with 15𝑁𝐻4𝐶𝑙15NH4Cl and D-glucose-1,2,3,4,5,6,6-d7 as the sole nitrogen or carbon sources. All media contained 100 mg/L ampicillin...
The speed of sound in sea water varies between 1480 (0.80 nm/sec) and 1560 m/s (0.84 nm/sec) depending on depth. Table Distance SOUS stations to sub Station Lon Lat Dist nm to 40N 180 Time mm:ss @ 0 km depth Time mm:ss @ 5.5 km depth Adak Wake Kaneohe Midway Adak 176.64 W ...
The aim here isto show that the boundary betweenthe informal and the formal was often porous and fluid in the recent history ofthe PRCasthe socialist state shiftedits policies in response to ideological con-testations,economic development strategies,aswell asresistance by workers.“Out-of-plan”...
WX AWOS-2 at 1O2 (16 nm SE): 118.35 MHz (707-262-0380) COMMUNICATIONS PROVIDED BY UKIAH RADIO ON FREQ 123.6 & 255.4. MHz APCH/DEP SERVICE PROVIDED BY OAKLAND ARTCC ON FREQS 127.8/353.5 MHz (UKIAH RCAG). Nearby radio navigation aids VOR radial/distance VOR name Freq Var --...
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针对stargardt病的药物治疗包括由alkeus公司开发的用于减少这种病中视网膜产生的“废物”的药物alk-001,目前这种药物治疗正在临床试验中进行测试。该药物仅针对这种疾病,而该疾病的发病率为1-5/10000(orphanet)。[0008]另一种替代治疗包括rdcvf,被鉴定为(léveillard等人,2004)在视杆即将死亡时保护视锥免于变性。[...