在棉花中通过VIGS干涉GhRING1表达,棉花对黄萎病抗性降低,验证了它在棉花抵抗黄萎病菌侵染中的作用.(3)通过生物信息学分析,在亚洲棉,雷蒙德氏棉,陆地棉和海岛棉中鉴定出108个PRA1基因.q RT-PCR结果表明,GhPRA1基因受Me JA,SA和ET诱导表达.GhPRA1.B1-1A过表达拟南芥植株对黄萎病菌的抗性增强,GhPRA1.B1-1A沉默...
小G蛋白矮化利用micro array技术对水稻幼苗在营养胁迫条件下根部基因表达的研究中发现:一个与豌豆Pra2(小G蛋白)基因有同源性的基因的RNA水平在营养胁迫后再补充营养时,表达量下降.用RT-PCR和PCR方法分别获得该基因的cDNA克隆-OsJPra2和该基因翻译起始位点上游1 kb的启动子序列.OsPra2基因编码的蛋白质具有结合GTP/...
On-target srp19-MO action was confirmed by RT-PCR, which demonstrated reduced levels of the PCR product corresponding to the normal transcript at all MO doses (supplemental Figure 9B-C), and also a MO dose–dependent increase in an aberrant PCR product corresponding to intron 3 retention, ...
• 探针法荧光定量RT-PCR试剂盒 +-生化试剂盒 • 生化检测试剂盒 • P450系列 • 氨基酸代谢系列 • 蛋白酶系列 • 蛋白含量系列 • 辅酶Ⅰ系列 • 辅酶Ⅱ系列 • 谷胱甘肽系列 • 氮代谢系列 • 淀粉系列 • 光合作用系列 • 果胶系列 ...
) on disease progression was determined by colony forming units (CFUs) in guinea pig lung following their aerosol infection and the expression levels of \{CXCL10\} and \{CXCL11\} were studied by quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR) and in situ RT-PCR. ...
根据政府规定进入普吉岛的旅客必须注册泰国通行码,并提供至少 10,000 美元的泰国医疗保险证明(仅限非泰国人),同时旅客必须提供接种满14天的疫苗官方证明,72个小时内的COVID-19 RT-PCR 检测结果为阴性将免于隔离。如未接种疫苗/未完全接种疫苗的人须接受5天的隔离,并在到达泰国4-5日接受1次 COVID-19 RT-PCR ...
Presence of mRNA transcripts, determined by RT-PCR, demonstrated that channel alpha- and gamma-subunits were similarly expressed in both groups but that beta-subunit mRNA was increased in PVL rats. This confirms that there was induction of rat ENaC and indicates that beta-subunit has a ...
and CDGp8R primers (Table 1; end concentrations for both primers 200 nM), 20 units of RNAse inhibitor (Invitrogen) and the components of the SuperScript III One-Step RT-PCR with Platinium Taq kit (12 μL of 2X reaction Mix and 2 μL of the SuperScriptTM III RT/ Platinium Taq Mix)...
a在模拟失重下,48 h后,检测ALP活性,用RT-PCR法检测OPG和BMP-2基因表达。 Under the simulation weightlessness, after 48 h, examines the ALP activeness, examines OPG and the BMP-2 gene expression with the RT-PCR law.[translate] a因为刚来日本,日语不是很好。刚开始找工作的时候,找了十几家都被拒绝...
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