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Full size image To determine the role of PRMT1 in CRC cell proliferation, two different short hairpin RNAs against PRMT1 were used to knock down PRMT1 expression in HCT116 cells. We found that PRMT1 expression in PRMT1-knockdown (PRMT1-KD) cells was reduced to ~ 20% of that in cell...
A genotype–phenotype correlation also holds in the sense that full loss of function of ARX results in the most severe presentation. In rodents, ARX encodes a transcription factor that acts downstream from Dlx1 and Dlx2, transcriptional regulators important in specifying interneuronal fate. ARX is...
Direct sequencing of the coding region identified the PRRT2 mutation c.649dupC in 5/5 families with infantile convulsions. The mutation was present in 23 family members, of which 18 were clinically affected and 2 were obligate carriers. The affected carriers of this mutation presented with diffe...
Full size image We then constructed an effector construct35S:ZmCPK39, which, along with the reporter construct35S:ZmDi19–LUC, was cotransfected into B73 protoplasts. Overexpression ofZmCPK39led to a significant decrease in ZmDi19–LUC compared to the empty vector control. Treatment with the prot...
G.E., A.C., V.G., J.C.M.W., S.R.H.The following authors had full data access and take responsibility for analysis:A.P., C.v.N., M.M., J.I.R., A.C., K.L.L, S.R.H.Cohort study investigators:a list of investigators by cohort study may be found in theSupplementary...
Not all PR-10 alleles will be expressed as a full-sized protein. 112 unique sequences had an intact ORF, while the remaining 22 sequences contain early stop codons or indels in the ORF that result in frame shifts followed by an early stop codon. The latter sequences were denoted as pseu...
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Full Name PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae) Calculated Molecular Weight 78 kDa Observed Molecular Weight 90 kDa, 78 kDa GenBank Accession Number BC000184 Gene Symbol PRPF3 Gene ID (NCBI) 9129 RRID AB_2170994 Conjugate Unconjugated Form Liquid Purification Method Antigen...
Full size image We next analyzed chromatin immunoprecipitation followed by sequencing (ChIP–seq) data in ChIPBase v.2.0 (ref.16) and observed the binding sites of relevant transcription factors within 5 kb upstream of thePRMT9transcription start site.CREB1, a known prognosticator17, showed the ...