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用50mM Tris.HCl[pH 7.6]、0.1%Triton X-100洗涤5遍后,通过于室温在样本缓冲液(1%SDS,50mM Tris.HCl[pH 7.6],10%甘油)中温育30分钟解离复合物并通过10%SDS-PAGE进行分离。利用mycl-9E10单克隆抗体在印迹上对PHO36MH蛋白进行免疫检测。 实施例2PHO36介导对渗透蛋白的敏感性。 利用GAL1-调节的cDNA表达文库...
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a Cas9/CRISPR-mediated gene-targeting strategy, we first generated mice with a Prrt2Stop mutation, in which two stop codons were introduced into exon 2 of the endogenous murine Prrt2 gene at a site correspond- ing to the C649 site of human PRRT2 (Supplementary information, Figure S3B)....
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We then attempted to examine the significance of PRRT2 interaction with components of the SNARE complex. Since assembled SNARE complexes are resistant to 1% SDS lysis buffer at room temperature but can be dissociated into monomeric SNARE proteins at 100 °C21,22, we compared the amount of assemb...
To complement the mass spectrometry, we performed phos-tag gel analyses in which SDS-PAGE gels are run in the presence of a molecule that binds to phosphorylated proteins, thereby causing a mobility shift. This approach dem- onstrated that the DP S-tag protein appears as...
1.5 提高姜黄素疗效的方法 不同的制造工艺和基质对姜黄素的溶解度,生 目前关于提高姜黄素药效的策略主要从 2 方面 物利用度等具有不同的影响.共沉淀法,微波淬冷 入手:(1)提升生物利用度,如对姜黄素结构进行 法,冷冻干燥法等为 SDs 常用的制备工艺[60].壳聚 改造,与抑制姜黄素失活或增加其缓释性的其他药 ...