将供试水稻样品在液氮中迅速研磨成粉末, 每0.1 g样品加入1 mL蛋白质提取缓冲液(62.5 mmol·L-1Tris-HCl (pH7.4), 10%甘油, 2% SDS, 1 mmol·L-1PMSF, 2 mmol·L-1EDTA, 5% β-巯基乙醇), 充分混匀后置于冰上10分钟, 期间每隔2分钟涡旋震荡30秒。4°C、13 400 ×g离心20分钟。取上清液(总蛋白)...
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Expression pattern and subcellular localization of PRRT2 in mouse brain.(A)Western blotting showing PRRT2 in the nervous system of adult mouse. GAPDH served as an internal control.(B)Western blotting showing the expression of PRRT2 in cultured primary neuronal rather than glial cells. NeuN was...
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Fig. 2: The NO–sGC–PRKG pathway modulates the contractility phenotype of VSMCs. a, bRepresentative images of F-actin staining (red) and DAPI-stained nuclei (blue) and F-actin quantification inaWT VSMCs treated as indicated for 5 min (n = 4 independent cell batches per condition...
a Cas9/CRISPR-mediated gene-targeting strategy, we first generated mice with a Prrt2Stop mutation, in which two stop codons were introduced into exon 2 of the endogenous murine Prrt2 gene at a site correspond- ing to the C649 site of human PRRT2 (Supplementary information, Figure S3B)....