FJ2-5-3 was 1770 bp and the encoded ADH2 enzyme consisted of 589 amino acids, with a signal peptide of 24 amino acids length present at the N-terminus and belonging to type I of the PQQ-dependent alcohol dehydrogenase family (Type I PQQ-ADH). The enzyme exhibite...
To validate the utility of the beads, we determined whether PQQ immobilized on the Sepharose beads maintains its cofactor activity. To this end, apo-GDH, which can be reactivated by PQQ, was incubated with the PQQ-Sepharose beads or EAH-Sepharose beads (control) for 30 min and GDH ...
酶(ADH)高表达,从而有助于葡萄糖醋酸杆菌(Gluconacetoba- cter europaeus)适应高酸环境。 3.2 促进植物生长、发育与繁殖 PQQ 能够缩短植物的花粉萌发时间,但是对花粉管生长 没有影响。PQQ 能够促进烟草种子萌发,提高脂肪酶活性和种 子呼吸速率。用PQQ 喷施烟草幼苗叶片并做灌根处理,发现叶 片和根系中吲哚乙酸、玉米...
Here, we show that the pyrroloquinoline quinone-dependent alcohol dehydrogenase (PQQ-ADH) activity of a commensal bacterium, Acetobacter pomorum, modulates ... SC Shin,SH Kim,H You,... - 《Science》 被引量: 578发表: 2011年 A Biofuel Cell with Electrochemically Switchable and Tunable Power Outp...
pasteurianus (pBBR1-p264), respectively. The catalytic activity of ADH and aldehyde dehydrogenase increased by 52.92% and 67.04%, respectively. The results indicated that the energy charge and intracellular ATP were also improved in the recombinant strain. The acetic acid fermentation was carried out...
Summary This chapter contains sections titled: Introduction Basic Technical Information Regarding Membrane-bound Enzymes Preparation of Cytosolic Fractions and Membrane Fractions EDTA Treatment of the Membrane Fraction Carrying PQQ as Coenzyme Assays of Enzyme Activity PQQ-Dependent Dehydrogenases Alcohol ...
The purified enzyme had maximum activity at pH 4.5 and showed the highest substrate specificity to ethanol, isoamyl alcohol, 1-butanol, and 1-propanol. The deduced sequences of cloned genes adhA and adhB encoding subunits I and II of PQQ-ADH showed 80 % amino acid (AA) identity to AdhA ...