FJ2-5-3 was 1770 bp and the encoded ADH2 enzyme consisted of 589 amino acids, with a signal peptide of 24 amino acids length present at the N-terminus and belonging to type I of the PQQ-dependent alcohol dehydrogenase family (Type I PQQ-ADH). The enzyme exhibite...
酶(ADH)高表达,从而有助于葡萄糖醋酸杆菌(Gluconacetoba- cter europaeus)适应高酸环境。 3.2 促进植物生长、发育与繁殖 PQQ 能够缩短植物的花粉萌发时间,但是对花粉管生长 没有影响。PQQ 能够促进烟草种子萌发,提高脂肪酶活性和种 子呼吸速率。用PQQ 喷施烟草幼苗叶片并做灌根处理,发现叶 ...
To validate the utility of the beads, we determined whether PQQ immobilized on the Sepharose beads maintains its cofactor activity. To this end, apo-GDH, which can be reactivated by PQQ, was incubated with the PQQ-Sepharose beads or EAH-Sepharose beads (control) for 30 min and GDH ...
Here, we show that the pyrroloquinoline quinone-dependent alcohol dehydrogenase (PQQ-ADH) activity of a commensal bacterium, Acetobacter pomorum, modulates ... SC Shin,SH Kim,H You,... - 《Science》 被引量: 578发表: 2011年 A Biofuel Cell with Electrochemically Switchable and Tunable Power Outp...
pasteurianus (pBBR1-p264), respectively. The catalytic activity of ADH and aldehyde dehydrogenase increased by 52.92% and 67.04%, respectively. The results indicated that the energy charge and intracellular ATP were also improved in the recombinant strain. The acetic acid fermentation was carried out...
Summary This chapter contains sections titled: Introduction Basic Technical Information Regarding Membrane-bound Enzymes Preparation of Cytosolic Fractions and Membrane Fractions EDTA Treatment of the Membrane Fraction Carrying PQQ as Coenzyme Assays of Enzyme Activity PQQ-Dependent Dehydrogenases Alcohol ...