MultiMam relies on small synthetic progenitor DNA plasmid modules, that are assembled into a multigene expression construct containing multiple expression cassettes by a procedure called tandem recombineering (TR)12. TR exploits sequence and ligation independent cloning for gene insertion iteratively coupled...
MultiMam relies on small synthetic progenitor DNA plasmid modules, that are assembled into a multigene expression construct containing multiple expression cassettes by a procedure called tandem recombineering (TR)12. TR exploits sequence and ligation independent cloning for gene insertion iteratively coupled...
Test your skills with a free sample quiz. Choose your discipline: PE Civil, PE Electrical, or PE Mechanical. What makes the questions difficult? Some questions are difficult because the pertinent theory is not obvious. There may be only one acceptable procedure, and it may be heuristic (or ...
Therapeutic procedureAcidssymptoms <1>酸类Proton pump inhibitor (PPI) is the first-line drug for GERD and is far more effective than H2-receptor antagonist (H2RA). H2RA suppresses mainly nocturnal gastric acid secretion from parietal cells, while PPI blocks acid production at nighttime as well as...
[53] A study may appear to show that a procedure is effective but it is found to be impossible to replicate that result. This appears endemic and if one of the assump- tions is that knowledge building proceeds by the gradual accumulation of empirical facts, the discipline is in real ...
We tested SENSE-PPI using other positive-to-negative ratios: 1:1 and 1:100, following the same procedure. We did not consider larger ratios, such as 1:1000, due to the ”neighboring-exclusion” condition, which restricts the availability of potential candidates for negative pairing at such ...
To propagate and maintain the pPIC6 and pPIC6/HSA plasmids, we recommend using the following procedure: 1. Use a small amount of the supplied plasmid stock solution in TE, pH 8.0 to transform a recA, endA E. coli strain like TOP10, DH5, JM109, or equivalent. 2. Select ...
The hypergeometric test52 is performed to calculate the statistical significance of having a given amount of success in a population. This procedure tests the statistical significance of the overlap between the proteomic and external datasets, while taking into consideration that only a subset of all ...
In this section, we describe the dataset and the modeling procedure used to develop both predictors. In both scenarios, protein–protein and protein–peptide systems, the performance of the models is evaluated with cross-validation and hold-out test sets. Data Collection: Protein–Protein Complexes...
dynamic analyzes the nonlinear relationship between CPI and PPI at different period by using step-test procedure.The results of nonlinear Granger causality test indicate that there exists a unidirectional nonlinear Granger causality from PPI to CPI during the sample period.What's more ，step-test ...