PP65 Antigenemia assay by indirect immunofluoroscence technique was carried out in all the cases. None of the cases showed positivity for the CMV antigen, though appropriately run control samples were all positive and the test is well established and standardized in our laboratory. This prospective ...
摘要:目的比较血 抗原和 抗体检测对移植患者巨细胞病毒( )活动性感染的诊断价值。方法收集 位移 植患者的血标本(共 份),分离血浆和多形核白细胞,血浆用于 抗体检测( ),白细胞用于 抗原血症检 测(荧光免疫组化)。同时随机取部分血浆标本进行荧光定量 分析,检测 。结果 抗原阳性细胞数与 拷贝数呈正相关(! ),...
CMVantigenemia.Methods Atotalof210EDTAanticoagulantplasmasamplesfrom36bo nemarrowor PeripheralStemCellTransplantPatientswereprospectivelycollectedfromSeptember1999tOApril2000. ThespecificCMVantibodyIgG/IgMofallpatientsweredetectedbyELISA.WedetectedCMVpp65 antigenemiabyindirectimmunofluorescenceassayusingCMVBriteKit.Allbloodsa...
CONCLUSION: Both the pp67-mRNA and the pp65 antigenemia assay predicted correctly the development of clinical HCMV disease in renal transplant recipients. However, the specificity of both tests with respect to development of HCMV disease, especially the pp65 antigen test was moderate. Significantly ...
·论 著·人巨细胞病毒 pp65 基因片段的原核表达及鉴定彭杰雄 1 ,谭洪 2 ,陆建国 2 ,林连成 2 ,邓兆享 1 ,林文浩 1 ( 1. 武警广东边防总队医院检验科,广东深圳 518029 ; 2. 深圳市赛尔生物技术有限公司,广东深圳 518055 ) 摘要:目的 利用基因工程方法表达人巨细胞病毒 pp65 基因片段,并对表达的目的蛋白...
versus pp65 antigenemia assay for monitoring c yto megal ovirus vire mia an d guiding p reempti ve gancrclovir therapy in al logeneic stem cell transplan ta ti on[J ] .Clin Mi crDbio1.20 0 1 .39 :3 9 3 8—3 9 4 1.
1. Comparison of a rapid cytomegalovirus pp65 antigenemia assay revealed by immunofluorescence to an in-house assay revealed by immunoperoxidase for diagnosis in solid organ transplant recipient patients.[J].Emerson; Carraro;Jacyr; Pasternak;Ana; Perosa;Itacy; Siqueira;Marines Dalla Vale; Martino...
We prospectively monitored 74 consecutive allogeneic and 50 autologous patients after bone marrow/stem cell transplantation from May 1999 to October 2000 at our institution with quantitative CMV PCR and pp65 antigen assay once weekly from conditioning th
Grefte, J.M. et al., “ Cytomegalovirus Antigenemia Assay: Identification of the Viral Antigen as the Lower Matrix Protein PP65,” The Journal of Infectious Diseases 166: 683-4, 1992. Lolli, Francesco et al., “T and B Cell Responses to Cytomegalovirus Antigens in Healthy Blood Donors and...
ofhumancytomegalovirusPp65antigenemiaamongrenaltransplantpatients[J].IndianJNephrol.2012,22(5):347–352. [3]EmersonC.,JacyrP.,AnaP., etal .ComparisonofarapidcytomegalovirusPp65antigenemiaassayrevealedbyimmunofluorescencetoanin-houseassayrevealedbyimmunoperoxidasefordiagnosisinsolidorgantransplantrecipientpatients[J]...