PP2A phosphatase activity assay 1 × 106SKM-1 cells were seeded into 6-well microtiter plates and treated with different concentrations of LB100 (0, 1.25, 2.5, 5, 10 μM). Following treatment for 6 hours, cells were washed twice with cold water, and lysed in RIPA buffer supplemente...
Due to the constitutive phosphatase activity present inside cells, a sensitive assay is required to detect the changes of PP2A activity under various experimental conditions. We optimized a fluorescence assay (DIFMU assay) by combining it with prior anti-PP2A immunoprecipitation to quantify PP2A-...
This study aimed to determine how cigarette smoke modulated the serine-threonine phosphatase protein phosphatase 2 A (PP2A) to alter the barrier function of human lung microvascular endothelial cells (HLMVECs). Cigarette smoke exposure lowered overall PP2A activity and enhanced endothelial permeability in...
PP2A Activity Assay—PP2A activity in immunoprecipitates of this enzyme was assayed using p-nitrophenyl phosphate as a substrate (phosphatase assay kit, Millipore). Briefly, the cells were lysed in coimmunoprecipitation lysis buffer (see above) at 4 °C. The lysates were centrifuged at 16,000 ×...
Amersham); the peak fraction (1.5 mg/ml) was incubated with 20 μM H2O2 at 37°C for 30 min and, after desalting, was diluted at least 100-fold into reaction buffer containing 25 mM Tris (pH 8.0), 150 mM NaCl, and 50 μM MnCl2 for the phosphatase activity and methylation assay. ...
PP2A activity was detected by assay kit and Ab level was also detected by ELIAS. Morris water maze and step-down avoidance tests were used to detected Tg2576 mice learning and memory abilities. Results Expression of pSUP-siI2PP2A or lenti-siI2PP2A decreased I2PP2A. SiI2PP2A increased PP2A ...
The applicability of this method is critically dependent on the lack of proteolytic activity which otherwise would cleave the peptide and remove the basic residues required for its binding to phosphocellulose. Methods based on colorimetric determination of inorganic phosphate released by the phosphatase ...
PP2A Immunoprecipitation Phosphatase Assay Kit 会员价请登录市场价￥3019 原厂型号17-313RF 贝雷猫货号RH169496 品牌默克密理博/Millipore 单件重量0kg 货期15天 单位:盒包装:盒库存:0 数量:盒+-1盒起订,增量1盒 累计成交0笔0条评价 商品名 贝雷猫货号 ...
SET knockdown enhanced PP2A activity. (A) PP2A phosphatase activity of CIP-m cells was analyzed as indicated in material and methods. Quantitative data from 3 independent experiments performed duplicate are shown. *P < 0.05 vs. shNT. (B) PP2A Tyr307 phosphorylation levels of CIP-m cel...
PP2A activity assay. PP2A activity was determined using the PP2A immunoprecipitation and phosphatase assay kit (Merck Millipore, Darmstadt, Germany) according to the manufacturer's instructions. Real-time RT-PCR. Total RNA was extracted using the RNeasy Mini Kit (Qiagen Australia, Doncaster,...