Recently, post-transcriptional gene silencing (PTGS) has been employed as a method of choice to control plant viruses. PTGS acts by cleaving or translational repression of target messenger ribonucleic acid (mRNA
But, recent research has evidently brought out the indispensible contribution of non-coding RNA in controlling and regulating gene expression. ncRNA such as siRNAs and microRNAs have been reported to greatly help in causing post-transcriptional gene silencing (PTGS) in cells through RNA interference (...
【答案】:序列特异性的RNA降解现象,是抵抗病毒侵染的一种反应。在植物中,由转染的DNA触发转录后基因沉默,称RNA共抑制(co-suppressic)n);在真菌中,称RNA压制;在动物中,称RNA干扰。
PTGS转录后基因沉默(post transcriptional gene silencing)一种首先在植物中确定,然后发现在动物中也存在的现象。尽管P… www.bioon.com|基于7个网页 2. 基因后转录的沈默作用 ...体内协助控制基因表现的一套系统,在早期研究中被称为基因后转录的沈默作用(post transcriptional gene silencing),目前在 … ...
Post-transcriptional gene silencing (PTGS) is a mechanism of gene regulation and defense against pathogens such as viruses. However, most plant viruses encode proteins called viral suppressors of RNA silencing (VSRs) which able to inhibit PTGS. Previously, BBWV-1 VP37 was identified as VSR and ...
Here we report that five independent transgenic lines having normal phenotype displayed characteristics typical of post-transcriptional gene silencing (PTGS): multiple copies of the transgene, low levels of the corresponding mRNA, methylation of the silenced transgene, and accumulation of p23-specific ...
of RNA viruses produce dsRNA intermediates, and they serve as inducers of post-transcriptional gene silencing (PTGS). Infection by DNA viruses also induces RNA-directed DNA methylation (RdDM), which is involved in modification of DNA and/or histones, result in transcriptional gene silencing (TGS)...
(2006). Efficient delivery of small interfering RNA to plant cells by a nanosecond pulsed laser-induced stress wave for posttranscriptional gene silencing. Plant Sci. 171:375-381.Wei Tang Douglas A. Weidner Benjamin Y. Hu Ronald J. Newton and Xin-Hua Hu.Efficient delivery of small ...
Posttranscriptional gene silencing (PTGS) in plants results from the degradation of mRNAs and shows phenomenological similarities with quelling in fungi and RNAi in animals. Here, we report the isolation of sgs2 and sgs3 Arabidopsis mutants impaired in PTGS. We establish a mechanistic link between...
3) Post-transcriptional gene silencing 转录后基因沉默 1. RNA interference(RNAi) refers to the process where by double-stranded RNA(dsRNA) induces sequence-specific post-transcriptional gene silencing(PTGS). RNA干扰(RNA i)是由双链RNA(doub le-stranded RNA,dsRNA)介导的、序列特异的转录后基因沉默...