The purification was done at room temperature (20°C) and the purity of the resulting cv-lysozyme 3 was determined by SDS-PAGE using a 12% acrylamide/bis gel in Tris-HCl buffer, followed by staining of proteins in the gel with 1% (w/v) Coomassie blue R-250 in 10% (v/v) acetic a...
The DinoSL-based e-cDNA library underwent single-molecule real-time (SMRT) sequencing using 6 SMRT cells on the PacBio Sequel platform. Sequencing was performed by BGI Genomics Co., Ltd. (Shenzhan, China). 4.5.3. Data Processing The raw SMRT sequencing reads were subjected to SMRT Analysis...
including Illumina, Element Biosciences, MGI Ultima Genomics, PacBio, and Oxford Nanopore, and the software can call both germline and somatic variants. One of the key benefits of