The pandemic likely acquired this nickname because of World War I, which was in full swing at the time. The major countries involved in the war were keen to avoid encouraging their enemies, so reports of the extent of the flu were suppressed in Germany, Austria, France, the United Kingdom...
CD3+T cells autologous to DCs were isolated from the CD14−fraction by magnetic-activated cell sorting-positive selection (MACS, Miltenyi Biotec). IFN-γ ELISPOT with the stimulated T cells was performed as described above. Non-radioactive and colorimetric based cytotoxicity assays were performed w...