Whether or not an Oncopig-derived cell line would be superior to the KRAS/SV40T cell line of the present report for mimicking PDAC in pigs is not known. So, the advantages of using wild type pancreatic tissue over Oncopig tissue to generate transformed cell lines for orthotopic implantation ...
OCM pro-143 Recombinant Human Oncomodulin-1 OLR1 pro-923 Recombinant Human Oxidized Low Density Lipoprotein Receptor 1 OMP pro-1412 Recombinant Human Olfactory Marker Protein Omp Pylori pro-369 Recombinant Helicobacter Pylori Outer Membrane Protein OmpA pro-571 Recombinant Bacterial Outer Membrane Protein...
PK15 cells response to the extracellular treatment of virus mimics dsRNA (PolyI:C), we detected and analyzed whole genome DNA methylation levels of PK15 cells treated by PolyI:C (P), DNMTs inhibitor (Aza-CdR, A), and both (P + A) by comparing to the untreated control cells (C)...
These cells are phagocytic and produce IFN-α in response to poly-I:C with similar kinetics as PAM, and support the replication of PRRSV and ASFV, representing an alternative to primary porcine macrophage cultures for cell–virus interaction studies, virus titration and detection, and for vaccine...
2.3. Virus Infection, Treatments with Chemicals and Transfection The PK-15 or PK-15/EGFP-LC3 cells were grown to 60% confluence in complete MEM at 37 ˝C and 5% CO2. Cells were then infected with PCV2 at a multiplicity of infection (MOI) of one and incubated at 37 ˝C and 5% ...
virus infection (12 DE-genes), measles (11 DE-genes), ubiquitin mediated proteolysis (11 DE-genes), prolactin signalling pathway (10 DE-genes), Salmonella infection (10 DE-genes), NF-kappa B signalling pathway (10 DE-genes), TNF signalling pathway (10 DE-genes), hepatitis C (10 DE-...
As a member of the ISGs, ISG12A can dramatically restrict different viral infections, such as hepatitis C virus (HCV), vesicular stomatitis virus (VSV) and Newcastle disease virus (NDV) [21–23]. Whether porcine ISG12A is involved in host resistance to PRRSV infection is of great ...
Keywords: arterivirus; porcine respiratory and reproductive syndrome virus; SARS-CoV-2; membrane protein; Gp5; M; Orf3a; protein structure; alphafold; palmitoylation 1. Introduction 1.1. Structure of Arteriviruses and the Function of Their Membrane Proteins Porcine reproductive and respiratory syndrome...
suis vaccine candidates in combination with the adjuvants Poly I:C, Quil A ®, Alhydrogel ®, TiterMax Gold ® and Stimune ®. The toll-like receptor ligand Poly I:C and the saponin Quil A ® polarized swine DC cytokines towards a type 1 phenotype, with preferential production of...