Modeling land-use change in the Ipswich watershed, Massachusetts, USA. Agriculture, Ecosystems & Environment, 85, 83–94. Google Scholar Shachar, A. (1998). Reshaping the map of Israel: A new national planning doctrine. Annals of the American Academy of Political and Social Science, 555, ...
Population structure and geographic distribution of the 384 accessions ofCerasussubgenus germplasm.AGeographic locations and fruit phenotypes of representative varieties from 384Cerasusaccessions. The size of each pie chart represents the sample size. The fruit images ofP. cerasoides,P. fruticosa, andP....
PCR reactions (20 μl total volume) contained 0.2 μM each primer, 0.2 mM each dNTP, 10 mM Tris-HCL pH 9.0, 1.5 Mm MgCl2 and 0.4 units of Taq polymerase (New England Biolabs, Ipswich, MA, USA) with cycle conditions: 95 °C for 5 min; 35 cycles at 94 °C...
(NEB, Ipswich, MA, USA). There were three biological repeats. Qubit 2.0 and Agilent 2100 were used to detect the concentration and insert size of the library. After the library was constructed, the inserts of the library were detected using the Qsep400 high-throughput analysis system, and ...
Amplification of the target fragments was carried out using Q5 high-fidelity DNA polymerase (NEB, Ipswich, MA, USA). The PCR products were purified with AMPure XP magnetic beads (Beckman Coulter, Indianapolis, IN, USA). The indexing of libraries was performed using the Nextera XT Index Kit ...
The high-throughput sequencing library construction procedure is briefly described as follows: (1) The genomic DNA was digested by 30 μL PstI-HF (8 units) and MspI (8 units) restriction enzymes (NEB, Ipswich, MA, USA) at 37 °C for 2 h. (2) The digested DNA fragments were examined...