Sepideh NematEstablished Products Janssen Research & Development UK High Wycombe UKSusumu NakaharaEstablished Products Janssen Pharmaceutical K.K. Tokyo JapanItalo PoggesiGlobal Clinical Pharmacology Janssen Research & Development Cologno Monzese ItalyJoseph Massarella...
Fifty milliliters of the sample extracts were injected into a UPLC (NEXERA X2 LC-30AD, Shimadzu Corporation, Kyoto, Japan) coupled to a time of flight high-resolution mass spectrometer (TripleTOF 5600+, AB Sciex, MA, US). The LC separation was performed on a XBridge BEH XP C18 column...
Viability was determined by 7-amino-actinomycin D (7AAD) uptake, and absolute cell counts were established using Flow-Count Fluorospheres (Beckman Coulter, High Wycombe, United Kingdom) according to the manufacturer's instructions. Cells removed at weeks 2 and 4 were used for cytogenetic or ...