The specificity of KASP markers were tested using two parent lines. PCR assays were conducted using a CFX 96 Real-Time System (Bio Rad, Hercules, CA, USA). A 5 μL reaction, including 2.5 μL KASP master mixture (LGC Genomics, Middlesex, UK), 2.4 μL 60 ng∙μL− 1...
Pooled Analysis and Writing (* equal contribution)—Bin Zhou (Imperial College London, UK); James Bentham (Imperial College London, UK)*; Mariachiara Di Cesare (Middlesex University, UK)*; Honor Bixby (Imperial College London, UK); Goodarz Danaei (Harvard T H Chan School of Public Health,...
A 5 μL reaction, including 2.5 μL KASP master mixture (LGC Genomics, Middlesex, UK), 2.4 μL 60 ng∙μL− 1 DNA, 0.04 μL 50 mM Mg2+ and 0.06 μL primer mix (primer A (100 μM): primer B (100 μM): primer R (100 μM): ddH2O = 12: 12: 30: 46), were ...
Pooled Analysis and Writing (*equal contribution)—Mariachiara Di Cesare (Imperial College London, London, UK; Middlesex University, London, UK)*; James Bentham (Imperial College London, London, UK)*; Gretchen A Stevens (World Health Organization, Geneva, Switzerland)*; Bin Zhou (Imperial Colleg...
Pooled Analysis and Writing (*equal contribution)—Mariachiara Di Cesare (Imperial College London, London, UK; Middlesex University, London, UK)*; James Bentham (Imperial College London, London, UK)*; Gretchen A Stevens (World Health Organization, Geneva, Switzerland)*; Bin Zhou (Imperial Colleg...