It is worth returning to the origins of modern considerations on local and global population changes. The seventeenth century saw the birth of the Scientific Method, the cognitive foundation of the Contemporary World16,17. Its enormous potential has been proven by the grand works of Isaac Newton,...
The Brucella broth containing the biopsy samples were vortexed in laboratory for 2 min and 200 µl of the mixture was streaked onto brain heart infusion agar (BHIA) plate containing horse serum (7 %, Invitrogen, Grand Island, NY, USA), IsoVitalex (0.4 %, Becton–Dickinson, San Jose, ...
gene, genomic DNA was PCR amplified and the resultant amplicons were then sequenced using ABI PRISM Big Dye Terminator Cycle Sequencing (Applied Biosystems, Grand Island, NY, USA) on an ABI 3730xl automated sequencer (Applied Biosystems; Figure 1 Geographical distribution of GNE variants in Iran....
Continental and island populations On the basis of its geographic range and migratory behavior, the Red-crowned Crane currently is divided into two entirely separate populations: continental and island populations. The island population lives in the southeast and northeast of Hokkaido, Japan. This pop...
The DNA libraries were labeled using PstI-adapters containing specific 9-bp barcodes. Libraries were sequenced using the Ion Proton PITM Chip (Life Technologies, Grand Island, NY, USA), according to the manufacturer’s instructions. QTL-seq analysis...
In brief, cells were washed and cultured with glucose-free medium containing 2-NBDG (20 μM, Invitrogen) or cultured with DMEM medium (Gibco, Grand Island, NY, USA) supplemented with 5% human AB serum (Gemini, West Sacramento, CA, USA) containing MitoTracker Red CMXRos (100 nM, ...
(2009). "Individual and geographical variability in the songs of wild silvery gibbons (Hylobates moloch) on Java, Indonesia," in The Gibbons: New Perspectives on Small Ape Socioecology and Population Biology, eds S. M. Lappan and D. Whittacker (New York, NY: Springer). 91-110....
(St.Louis, MO, USA). RPMI-1640, foetal bovine serum and gentamicin were bought from GIBCO BRL Life technologies (Grand Island, NY, USA). The proteinase K, the sodium dodecyl sulfate (SDS), ribonuclease (RNase), Sarkosyl, Thiobarbituric Acid (TBA), and pyridine were purchased from Sigma ...
The tissues were then mounted with Prolong Gold mounting medium with DAPI (Life Technologies; Grand Island, NY). Images were taken using an epifluorescence Nikon Eclipse Ti-E inverted microscope (Melville, NY). Macrophage depletion using intraperitoneal injection of clodrosome and imaging using ...
Analysis of genetic relationship among isolates using Provesti’s distance exhibited significant bootstrap support to separate the main group of isolates from North Carolina, which were collected from different fields across the state (Fig.2). The other group comprised isolates from SC, TN, NY, GA...