All PCRs were performed in 10 µl volumes containing 10–200 ng of genomic DNA, 200 nM dNTPs (Promega, Annandale, NSW, Australia), 25 ng of each primer, 1× ReddyMix buffer (Abgene, Epsom, Surrey, UK) and 0.2 units of Thermo- prime Plus DNA Polymerase (Abgene). PCR amplification...
All PCRs were performed in 10 μl volumes containing 10–200 ng of genomic DNA, 200 nM dNTPs (Promega, Annandale, NSW, Australia), 25 ng of each primer, 1× ReddyMix buffer (Abgene, Epsom, Surrey, UK) and 0.2 units of Thermoprime Plus DNA Polymerase (Abgene). PCR amplification ...