hRPE cells were isolated from neonatal human globes and cultured on PolyHEMA and polystyrene substrates (as controls) in 24-well culture plates. DMEM/F12 was supplemented with 10% fetal bovine serum (FBS) and/or 30% human amniotic fluid (HAF) for cultured cells on polystyrene and PolyHEMA ...
Each mixture was immediately poured into a contact lens mold or between two PTFE plates separated by a 2 mm thick spacer. After 24 h of polymerization at 70 °C, the solids were immersed in water several times to remove residual unreacted agents and obtain the hydrogels. The compositions of...
During the albumin elution, theoretical plate numbers up to 30,000 plates/m were achieved by increasing the salt concentration.doi:10.1007/s00216-005-0121-7Ender UnsalAysun DurduBegum ElmasMurvet TuncelAli TuncelAnal Bioanal ChemAnalytical and Bioanalytical Chemistry...