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In the second stage of polycondensation, the pressure was gradually reduced to 5.0 Pa over a period of about 30 min, to remove the excess diol, to avoid excessive foaming, and to minimize oligomer sublimation, which is a potential problem during melt polycondensation. The temperature was ...
The pastes were lyophilized in a Christ-Alpha 2-4 LD lyophilizer (Martin Christ, Osterode, Germany) for 1–2 days, depending on the sublimation rate of the aqueous buffer. The resulting immobilized enzyme derivatives, obtained as powders, were stored at 4 ◦C under vacuum in a desiccator ...
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