Cleavage and Polyadenylation Specificity Factor (CStF) is a factor involved in the endonucleolytic cleavage process during transcription in eukaryotic organisms. It increases the efficiency and specificity of cleavage, but is not directly involved in polyadenylation. ...
The wild-type Sen1 helicase domain (residues 1095 to 1904) was amplified from the FL gene using PCR and was transferred into the 1-O vector (Addgene, plasmid 29658) by ligation-independent cloning53. The construct contained an N-terminal 6xHis-Mocr tag followed by a TEV cleavage site. Th...
RNA sequencing of fibroblasts induced into quiescence by contact inhibition reveals downregulation of genes involved in mRNA processing, including splicing and cleavage and polyadenylation factors. These genes also show differential exon use, especially increased intron retention in quiescent fibroblasts compared...
For instance, the RNA recognition motif (RRM)-containing protein Hrp1, the only member of the yeast cleavage factor IB (CFIB) subunit, does not have a mammalian or plant homolog [27, 28]. Therefore, whether plants and mammals have evolved new factors analogous to Hrp1 is unclear. Here, ...
Rao S, Dinkins RD, Hunt AG (2009) Distinctive interactions of the Arabidopsis homolog of the 30 kD subunit of the cleavage and polyadenylation specificity factor (AtCPSF30) with other polyadenylation factor subunits. BMC Cell Biol 10:51. : 10.1186/1471-2121-10-51 Central...
Cleavage factor polyribonucleotide kinase subunit 1 cMET: MET proto-oncogene CPSF: Cleavage and polyadenylation factor CSTF: Cleavage stimulation factor CTD: Carboxy-terminal domain CTNNB1: Catenin beta 1 DHFR: Dihydrofolate reductase DSRM: Double-stranded RNA-binding motif ...
These include CPF (Cleavage and Polyadenylation Factor) and CF1 and 2 (Cleavage Factor 1 and 2; note the yeast CF complexes differ from the mammalian ones, and that the differences are matters of terminology and not function; [2]). While biochemical fractionation and purification has led to ...
FCA mediates poly(A) site selection by physically interacting with the cleavage and polyadenylation machinery via an interaction with FY (Quesada et al., 2003, Simpson et al., 2003), the homolog of S. cerevisiae Pfs2p and human CPSF WDR33 (Ohnacker et al., 2000, Shi et al., 2009)....
It has been shown both in mammals and in plants that alterations in various polyadenylation factors resulted in significant changes in poly(A) site choice. These studies demonstrated the roles of Poly(A) Binding Protein Nuclear 1 (PABPN1), Cleavage Factor I (CF I), Cleavage and Polyadenylation...
Van Dijk et al., “Identification of RNA sequences and structures involved in site-specific cleavage of IGF-II mRNAs,” RNA, 1623-1635, 1998. van Ooij et al., “Polyadenylation of genomic RNA and initiation of antigenomic RNA in a positive-strand RNA virus are controlled by the same ci...