Notes Enantioseparation of N-Dansyl-DL-amino Acids by Polyacrylamide Gel Zone ElectrophoresisENANTIOSEPARATIONPOLYACRYLAMIDE GEL ZONE ELECTROPHORESISBETA-CYCLODEXITRINNORMAL-DANSYL-AMINO ACIDEleven known samples of mixed powder of cocaine and sodium hydrogencarbonate were measured by an X-ray diffractometer; ...
Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility. ...
g.Protein dye migration is not evenGel is partly insulated by air bubbles.Remove air bubbles before electrophoresis Insufficient coolingImprove the cooling or run at a low current h.The protein band-lane broadens at the bottom of the separation gelSample density...
The use of polyacrylamide as gel media for electrophoresis makes it the most commonly in situ synthesized material [2]. Moreover, using functionalized acrylamides as monomers, it is possible to produce hydrophilic [3], or hydrophobic gels [4], thermosensitive (“smart”) materials [...
RNA quantity and integrity were estimated by agarose gel electrophoresis in 1× TAE buffer. Complementary DNA (cDNA) synthesis was performed with a RevertAid First Strand cDNA synthesis kit (Thermo Scientific, Waltham, MA, USA) with oligod (T) primers (Evrogen) according to the recommendation ...