具体而言,作者选取了合适的分支多聚A尾的合成策略,再通过筛选获取不能被水解的非自然分支连接,在对不同类型的寡核苷酸交联化学进行测试后,作者确定了铜催化叠氮-炔环加成反应 (CuAAC) ,并引入高效液相色谱进行分支RNAs的纯化。 分支mRNA的合成和筛选 接下来,文章解析了分支多聚A尾增强mRNA翻译能力的机制。作者将蛋...
这种纯化方法需要梯度洗脱,寻找能够将circRNA和杂质线性RNA分子分离开来的最佳洗脱条件。 2024 年 12 月 27 号,郑州大学基础医学院梅英武团队在bioRxiv上传预印本文章:A poly(A) polymerase and oligo(dT)-dependent method for thepurification of en...
这种纯化方法需要梯度洗脱,寻找能够将circRNA和杂质线性RNA分子分离开来的最佳洗脱条件。 2024 年 12 月 27 号,郑州大学基础医学院梅英武团队在bioRxiv上传预印本文章:A poly(A) polymerase and oligo(dT)-dependent method for thepurification of engineering circular RNAs that enhances proteinexpression in eukaryo...
Total RNA was extracted from human kidney or human embryonic kidney cells in culture, then passed through oligo-d(T) cellulose to obtain total polyA(+)RNAs. Messenger activity of the preparations was checked in vitro using the rabbit reticulocyte lysate system and peptides synthesized in vitro ...
在细胞核中存在多种类型的RNAs,例如tRNAs,U snRNAs,mRNAs以及tRNAs,不同类型的RNAs拥有不同的出核途径,不同的出核途径会影响RNA在细胞质中的命运,这意味着RNA出核机器必须可以区分RNAs类别,每一种RNA应当有指示其出核途径的鉴别特征。转录本上存在适度长度的polyA尾巴是mRNA出核的身份认证,即便去除polyA尾巴的转录...
Chemical Biology发表文章：Chemically Modified mocRNAs for Highly Efficient Protein Expression in ...
与这些类似一致,若干 SEP1 互相影响的蛋白质,包括 ZC3H18, ARS2, Acinus 和 Brr2,为 polyA RNAs 的有效核出口需要。 翻译结果3复制译文编辑译文朗读译文返回顶部 符合这些相似之处,几个 SEP1 相互作用的蛋白,包括 ZC3H18、 ARS2、 腺泡和 Brr2,所需的有效核出口的 polyA Rna。 翻译结果4复制译文编辑译文...
(Polymerases I, II, and III), each responsible for transcribing different types of RNA: Polymerase I is responsible for ribosomal RNA, Polymerase II is responsible for mRNA and miRNAs, and Polymerase III transcribes tRNA and other short RNAs. Although not as well studied as prokaryotic ...
(A) negative RNAs and rRNA, respectively. The poly(A)-selected protocol enriches poly(A) + transcripts including mRNAs and many non-coding RNAs5,6, and also reduces the amounts of pre-mRNAs. It has become a widely used RNA-seq protocol thanks to its low-noise rate. In contrast, ...
(A) tail of t-PA mRNA and its translational activation. Furthermore, by comparing wild-type and mutated RNAs, Stricklandet al.found that preventing poly(A) addition to the t-PA mRNA inhibited translation activation (Stricklandet al.1988). These studies strengthened the idea that poly(A) ...