5. Rinse the culture surface 3 times with a large volume of distilled water (e.g., 100 µL/well for a 96-well plate), making sure to rinse the culture vessel thoroughly, as excess Poly-D-Lysine solution can be toxic to the cells. 6. Remove the final wash and leave the coated ...
384孔板,Corning Low Volume 384 Well Black with Clear Flat Bottom Polystyrene Poly-D-Lysine Coated Microplate, 10 per Bag, with Lid, Aseptically Manufactured,3643,Corning,康宁 市场价:询价/ 箱 在线询价 优惠:请询价 订货号:5RX8033 品牌型号:康宁 3643...
Airway epithelial cells in primary culture were seeded at 1.2 × 105 on 25-mm collagen-coated glass coverslips and grown 48 h in SAGM medium prior to transfection. CF/T43 cells were seeded at 1.2 × 05 on 25-mm glass coverslips and grown for 24 h in KGM medium (Clonetics) prior to ...
tdASC were plated in cell culture dishes coated with gelatin, laminin, collagen I (Col I), Matrigel, laminin/collagen I (Lam/Col), or laminin/poly-d-lysine (Lam/PDL). After we seeded the tdASC and ADSCs on these substrates, we conducted the transfection process described above followed ...