The phbC gene encoding the third enzyme of the poly-beta-hydroxybutyrate biosynthetic pathway, poly-beta-hydroxybutyrate polymerase, in Alcaligenes eutrophus H16 has been identified by the complementation of poly-beta-hydroxybutyrate negative mutants of A. eutrophus H16. These results demonstrate that ...
Identification and characterization of the PHB polymerase gene (phbC). J. Biol. Chem. 1989, 264, 15298–15303. [PubMed] 13. Yu, H.M.; Yin, J.; Li, H.Q.; Yang, S.L.; Shen, Z.Y. Construction and selection of the novel recombinant Escherichia coli strain for poly(beta-...
[24]. PCR experiments were performed using GoTaq DNA polymerase (TaKaRa, Kusatsu, Japan) with corresponding primers. Positive transformants were verified by colony PCR and Sanger sequencing. 2.3. Reconstruction of Expression Vector Two T7 promotors in the expression vector pACYCDuet-1 were ...