Primerswere once a standard part of every child's education. The firstprimerprinted in North America, The New EnglandPrimer, was typical; it contained many quotations from the Bible and many moral lessons, and the text was accompanied by numerous woodcut illustrations. We no longer use the wo...
② +er(名词后缀)= primer ③ +ate(名词后缀)= primate ④ +ord(开始,成形)+al(形容词后缀)= primordial POLY 表示“many” polypous ['pɑlɪp] n. 息肉;珊瑚虫;水螅虫 polyglot ['pɑlɪɡlɑt] n./adj. 通晓数种语言的人 polymer ['pɒlɪmə] n. 聚合物 polygraph ['pɑl...
第一步以poly(T)作为引物对mRNA3′端克隆。当新和成cDNA延伸到mRNA5′帽子结构时,加入莫洛尼鼠白血病病毒逆转录酶(MMLV)在cDNA3′端加入若干胞嘧啶poly(C)。MMLV所催化的加尾反应需要依赖模板和帽子结构的存在,因此只有完整的cDNA末端才会加上胞嘧啶残基,接着加入特异性引物,该引物在3′末端含有含有多聚鸟嘌呤核苷...
HeLa Total RNA and Control Human Actin PCR Forward Primer to verify components and protocol. Store reagents at -15°C to -30°C in a non-frost-free freezer. Store HeLa Total RNA at -80°C. 图表 Fig. 2, Human actin Poly(A) comparison ...
组分 T427S T427L Poly(A) Polymerase(黄色盖) 20μL 60μL 5× Poly(A) Polymerase Buffer(白色盖) 40μL 120μL PreScript II Enzyme Mix(绿色盖) 40μL 120μL 5× PreScript II RT Buffer(红色盖) 80μL 240μL Universal RTase Primer(透明盖) 45μL 130μL 2× FQ miRNA SYBR qPCR Pro...
首先根据mRNA3′末端天然存在的Poly(A)尾部设计反转录引物逆转录获得第一条cDNA链。根据已知的cDNA序列设计基因特异性引物(gene specific primer,GSP)合成第二条cDNA链。随后以基因特异性引物(GSP)及正义链3′末端引物作为一对引物,对得到的cDNA链进行PCR扩增,从而得到cDNA的3′端序列(基因特异性引物→3′末端)。
Poly(A) 尾长测定试剂盒 使您仅用三个小时即可快速测量多个 mRNA 的多聚腺苷酸尾。该试剂盒采用创新性 G/I 延伸技术,既保留了 RNA 的 3' 端,又能保证准确测量多聚腺苷酸尾的长度。而且,各种反应成分均经过优化,所以您可以快速获得高质量数据。描述:Poly(A) 尾长测定试剂盒通过四个主要步骤即可测定多聚...
Fig 1. Scheme for stem-loop poly(A) tailed RT-PCR.1: Poly(A) tailing of sncRNA using poly(A) Polymerase and ATP. 2: Reverse transcription of poly(A) tailed sncRNA primed by auniversal stem-loop Poly(T) RT primer. 3: First-strand cDNAs of poly(A) tailed sncRNAs are amplified in...
PolyesterPrimer Longpotlife,superiorflowandlevelling,combinedwith theeasiestsandingofanyprimer.Easyscraping. SelectAPrimerForYourWorkFlow Don’talwaysmistake“economy”fortheonlyprimingsolution.Ourprimershave beendesignedinconjunctionwithprofessionalspraypainterstoofferparticular ...
We herein describe a simple, sensitive and specific method for analysis of circulating microRNAs (miRNA), termed S-Poly(T) Plus real-time PCR assay. This new method is based on our previously developed S-Poly(T) method, in which a unique S-Poly(T) primer is used during reverse-transcript...