The N-terminal region of PrP is highly flexible, containing poly (-proline) type II (PPII) helical content. The five octarepeat sequences in the N-terminal region of the prion protein (amino acids 53–90) are also involved in metal ion binding. While the biological role of the N-...
These inhibitors feature a phosphonate ester or phosphinic moiety in place of the scissle bond. These groups inhibit MMPs, and methods been developed for their convenient incorporation within a peptide sequence by solid-phase methods.Gregg B. FieldsRobert HammerUS...
1990 . Helix-coil stability constants for the naturally occurring amino acids in water, XXIV: Half-cystein parameters from random poly (hydroxybutylglutamine-co- l -proline) . Biopolymers 30 : 121 – 134 .Wojcik J , Altman KH , Scheraga HA . 1990 . Helix-coil stability constants for the...
in addition to Arg88, also Arg136 in the carboxy terminal helix has a major contribution to PI(4,5)-P2-binding. Given that mutant R136D, but not R88A, displays wild type actin binding activity, we propose that the PI(4,5)-P2and actin...
Moreover, “ΔV for the coil-helix transition [of poly-L-glutamic acid, PLGA] is in the order of 1 ml. per amino acid residue” [54]; such an extremely small value of ∆V¯tr is surprisingly comparable to that of galacturonan, in spite of the large structural difference of the...
Myeloproliferative disorders (MPD) constitute a subset of hematological malignancies characterized by a stem cell-originated clonal proliferation of aberrant myeloid cells, one of which is polycythemia vera (PV). PV is the most common myeloproliferative disorder with an annual incidence of at least 23 ...
Based on the helix propensity scale, positively charged amino acids (K/R) have a tendency to form α-helices (Pace and Scholtz, 1998). Since PBMs are located in lysine- and arginine-rich regions, it would be likely to find several of them in a helical conformation. A summary of pADPr...
Therefore, it is expected that the high-molecular-weight poly(L-valine) does not take an extended conformation like the /3-sheet type, but rather an a-helix or similar helical structure. Including the present polypeptide, the a-axis dim- ensions of the unit cells in a-polypeptides are ...
Approaches to utilize the ectodomains of single membrane-spanning type I cytokine receptors such as the TNF or IL-6 receptor have been successfully developed as immunomodulatory drugs7, 41. However, mimicking the ectodomain of seven-helix membrane-spanning GPCRs is inherently complex due to the ...
Still further, triple helix molecules can be utilized in reducing the level of target gene activity. These techniques are described in detail by L. G. Davis, et al., eds, Basic Methods in Molecular Biology, 2nd ed., Appleton & Lange, Norwalk, Conn. 1994. In another alternative, the ...