a stock solution by dissolv ing 25 mg/ml poly ly sine in 4.73 ml water (both poly-L-lysine an d poly-D-lysine are used to coat tissue culture surf aces; check specif ic protocol f or choice of isomer) and f ilter sterilize throu gh a 0.22-μm f ilter. Store in 100-μl ...
十一、多聚赖氨酸(poly-l-lysine):常用包被培养皿的基质之一.。 1.配制硼酸缓冲液(PH8.4) A液:硼砂溶液--1.907g溶于100ml三蒸水(0.05M) B液:硼酸溶液--1.237g溶于100ml三蒸水(0.2M) 4.5mlA液+5.5mlB液=硼酸缓冲液(PH8.4) 2.多聚赖氨酸5mg溶于50ml硼酸缓冲液中,配成100μg/ml的多聚赖氨酸溶液。
Poly-L-lysine的中文名为多聚赖氨酸简称PLL,分子式为L-Lys-(L-Lys)n-L-Lys·xHBr,分子量为150,000-300,000。Poly-L-lysine多聚赖氨酸是通过链霉菌属的细菌菌株自然发酵生产聚赖氨酸。链霉菌(Streptomyces albulus)最常用于科学研究,也用于ε-聚赖氨酸的商业生产。α-聚赖氨酸通过碱性缩聚反应合成制备。 赖...
另外,有本书上是这么写的,供参考:Poly-lysine-coatedtissueculturesurfacesTocoatcoverslips:Prepareastocksolutionbydissolving25mg/(bothpoly-L-lysineandpoly-D-lysineareusedtocoattissueculturesurfaces;checkspecificprotocolforchoiceofisomer)--μlaliquotsat?20°,diluteonealiquotin40mlwatertoprepare13μg/,then...
多聚赖氨酸Poly-L-Lysine配制保留应用总结最新 系统标签: 赖氨酸多聚lysine配制poly渭砚 托梗犁嘉防撰焦灼燕岔立榷媳磁马熬分姓曹溶胺楔左鹰臭戳壁奏英狗树泵监臻辽境踩谐酚跪沪贾锑恢篇师琅专柑尉转寝捧悔响拿蚊召忍奢磕噶谓线铃租哮颖螺鳖窟舞羌护聊骆鞋在愿佣篡锭虚丘瞄蝶李哄人瓮扮功焊赞昂淮...
Cells were also transfected by poly( l-lysine) and liposomal carriers, and increases in secreted tPA similar to those seen with PEI-mediated transfection were observed for positively transfected cells. The results of these investigations indicate that non-viral gene delivery can induce a state of ...
Poly-l-lysine-coated magnetic nanoparticles as intracellular actuators for neural guidance It has been proposed in the literature that Fe3O4magnetic nanoparticles (MNPs) could be exploited to enhance or accelerate nerve regeneration and to provid... R Vittoria,C Riggio,Calatayud,... - 《Internation...
The objective of this work is to engineer a self-protected medication using a polymeric nanoparticle assembly of a biocompatible polymer, poly (lactic-co-glycolic acid) (PLGA), a free radical scavenger molecule (Trolox) and a linker poly-l-lysine (PLL) (Fig. 1a). Melatonin is used as ...
Acetylation occurs on two residues in α-tubulin: on a highly reactive lysine (HRL, Lys 394; Szasz et al., 1986) in brain microtubules and on the ∈-amino group of Lys 40 in α-tubulin (Greer et al., 1985; L’Hernault and Rosenbaum, 1985a,b). Acetylation of Lys 40 is a marker...
% poly-L-lysine solution for ˜30 min and then the mica was rinsed five times with DI water and dried in a nitrogen stream. About 80 μL of a de-ionized (DI) water diluted suspension of CNCs (1:10000) was deposited onto the substrate and incubated for ˜1.5 min. The samples ...