方法细胞分为对照组、Poly(I：C)组、苦金片组(1mg/ml)o Poly(I：C)组予20|ig/ml Poly(I：C)刺激12h,苦金片组在Poly(I：C)刺激后予苦金片(1mg/ml)刺激6h,对照组不予特殊处理。检测各组抗病毒因子［Toll样受体3(TLR3),干扰素调节因子3(IRF3),p干扰素(IFN-P),干扰素刺激基因15(ISG15),寡腺昔酸合成...
Poly(I∶C)组予20μg/ml Poly(I∶C)刺激12 h,苦金片组在Poly(I∶C)刺激后予苦金片(1 mg/ml)刺激6 h,对照组不予特殊处理。检测各组抗病毒因子[Toll样受体3(TLR3),干扰素调节因子3(IRF3),β干扰素(IFN-β),干扰素刺激基因15(ISG15),寡腺苷酸合成酶(OAS1),黏病毒抗性蛋白1(MX1)],炎症因子[白细胞...
研究人员发现经 poly(I:C) 处理后,TRIM25 WT 能显著增强 IFNα、IFNβ、IFNγ 和 ISG56 的表达。相反,转染 TRIM25 PTFGAAAA或∆PTFG后,这些 IRF3 依赖性基因的表达则不被增强(图4)。 图4. TRIM25-G3BP1 共相分离激活RIG-I介导的先天免疫 (A-D) 用 TRIM25 WT、TRIM25 PTFGAAAA或∆PTFG转染H...
Combination treatment of cells with both bLF and polyinosinic-polycytidylic acid increased mRNA and protein expression of several IFN genes (IFNB, IFNL1, and IFNL2) and IFN-stimulated genes (ISG15, MX1, IFITM1, and IFITM3) in Caco-2 cells. However, treatment with bLF alone did not induce...
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c Expression of poly-GA and poly-PR mRNA is comparable between GA-Nes and affected PR-Nes mice, but not asymptomatic PR-Nes (AU = arbi- trary units).[ANOVA F(3,16) = 16.2 p < 0.001 η2 = 0.753, Dunn's post hoc Nes (1.1 ± 0.51) vs GA-Nes (42 ...
Polymeric chains of nitrogen were found in the high-pressure C2/c phase of CsN2. This structure contains a substantially high content of single N-N bonds that exceeds the previously known nitrogen chains in pure forms, and also exhibit metastability at ambient conditions. We also identified a ...
Zur Erforschung m~glicher Besiedlungspr~iferenzen yon Pomatoceros triqueter unterschie- den sich die Platten voneinander (a) durch ihr Herstellungsmaterial (PVC, Plexiglas oder Glas), (b) durch ihren Helligkeitsgrad (schwarz, rot oder transparent) sowie (c) durch ihre Oberfl~ichenstruktur...
1c–e). Similarly, knockdown of PARP9 markedly reduced production of type I IFN, but not IL-6, by THP-1 macrophages compared to cells treated with control shRNA (sh-Ctrl) (Fig. 1c–e). To determine the role of PARP9 in primary cells, we first analyzed expression of PARP9 in ...
Therefore, the type I and type III interfaces were of critical importance for tetrameric CARDs complex formation. The intermolecular interaction between A-D subunits was much less extensive than that of the other types of interfaces between A-B, B-C, and C-D subunits (Figure 2B). To ...