parental SW620 and isogenic cells were seeded at 1.5 10 4 cells/well into 96-well Ultralow adhesion plates (ULA; Costar, Corning, Inc., Corning, NY), plates that we coated with polyHEMA as described =-=(22)-=-, or standard tissue culture treated plates (Falcon, Becton Dickinson...
The concentrated material is separated on a Pharmacia Superdex-200 gel filtration column, whereafter the active fractions (4 ml volumes) are pooled, treated with PMSF (1 mM) and dialysed in 10 mM Tris-HCl (pH 8.0) for 18 hours. The pooled active fractions are loaded onto a Mono Q anion...
parental SW620 and isogenic cells were seeded at 1.5 10 4 cells/well into 96-well Ultralow adhesion plates (ULA; Costar, Corning, Inc., Corning, NY), plates that we coated with polyHEMA as described =-=(22)-=-, or standard tissue culture treated plates (Falcon, Becton Dicki...
Hemaida, A.; Ghosh, A.; Sundaram, S.; Mallick, T.K. Evaluation of thermal performance for a smart switchable adaptive polymer dispersed liquid crystal (PDLC) glazing. Sol. Energy 2020, 195, 185–193. [Google Scholar] [CrossRef] Saeed, M.H.; Zhang, S.; Cao, Y.; Zhou, L.; Hu...
After this grinding process, the thickness of the plates was 2.2 ± 1 mm. Square pieces measuring 12 ± 1 mm on a side were cut from the plates for the microbiological test, and rectangular pieces measuring 10 ± 1 × 20 ± 1 mm were cut for the SEM investigation. The edges and ...
The plates were incubated for 3 h. The fluorescence of the reduced dye was determined by using a flatbed spectrofluorimeter Tecan Infinity F200 (Tecan, Männedorf, Switzerland) (λexcitation = 530 nm, λemission = 590 nm). In order to determine the cytotoxicity of materials, the direct ...
showed the capability for preparing different metal oxides embedded in PVA structure in just a one-pot method by ablating metal plates (Al, Cd, and Cu) in PVA solution [40]. Moreover, A. Mostafa et al. showed another facility laser parameter to enhance the PLAL method, by making laser ...
challenges Article Measurement of Poly(ADP-ribose) Level with Enhanced Slot Blot Assay with Crosslinking Yuko Kudo 1,2, Yuka Sasaki 3,4, Takae Onodera 3,4, Jun Hashimoto 5, Tadashige Nozaki 6, Kenji Tamura 5, Masatoshi Watanabe 2,† ID and Mitsuko Masutani 1,3,4,* 1 Genome Stability...
As AlamarBlue™ can interact with the cell culture medium, resulting in inconsistent fluorescence signal [51], P(HEMA-AEMA) cubes without the cells, but treated according to the same protocol, were used as a negative control. After the incubation with the reagent, the scaffolds were washed ...