cell attachment Your guide to using Gibco Poly-D-Lysine for maximum cell attachment and adhesion Introduction Fastidious cells such as primary rodent neurons and human pluripotent stem cell (PSC)–derived neurons require the coating of poly-D-lysine (PDL) to attach, spread, and grow in vitro. ...
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FITC-labeled lactosylated poly-L-lysine was added dropwise to the rhodamine-labeled plasmid in a 4:1 (wt/wt) ratio and held for 30 min at ambient temperature. The complex was suspended in DMEM (GIBCO BRL, Gaithersburg, MD). When applicable 100 or 50 μM chloroquine (Sigma, St. Louis...
Minimal essential medium (MEM), Dulbecco's modified Eagle medium (DMEM), RPMI-1640, L-15, phosphate buffered saline (PBS), L-glutamine, and penicillin/streptomycin were obtained from GibcoBRL (Gaithersberg, MD). Mouse recombinant IL-3 was from R & D Systems (Minneapolis, MN). B16-BL6,...
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PARP1 Inhibition Decreases LPS- Mediated HMGB1 Acetylation The secretion of HMGB1 occurs via a nonclassic secretory pathway, and hyper- acetylation of several lysine residues dis- tributed throughout the molecule is a vital step prior to its release. As a next step, we sought to determine ...
aCollected from Sigma-Aldrich, b–dCalculated using 1H NMR spectra, and eMeasured from GPC. Figure 3. (a,b) Sol-gel phase transition photographs of PEG-PACU copolymers (25 wt%) as a function of pH and temperature and (c) Acid-base titration curve of PEG-PACU copolymers. GenPcCTe...
The BioID assay is based on the expression of a promiscuous biotin ligase fusion protein that catalyzes the biotinylation of proximate lysine residues, to allow for the identification of proximal and interacting proteins in the context of living mammalian cells [80]. Poly-GA, poly-GR, and poly...