Product search for: Poly BSA Categories based on Poly BSA: adduction anhydride concentrate pibsa polyisobutylene succinic thermal type IUPAC name CAS EINECS Formula Poly BSA Poly BSA Type I Poly BSA Type II T2007B Polyisobutylene Succinic Anhydride(Thermal Adduction PIBSA) T2007A Polyisobutyle...
using a linear gradient between 0.3 and 2.0MKCl (7.5 column volumes). Add methylated BSA (0.1mg/ml) to each fraction as soon as they are collected. Active fractions (eluting between 0.7 and 2.0MKCl) are identified byin vitrodeadenylation, using the RNA substrate L3(A30) radioactivel...
After washing, non occupied plastic is blocked with 5% BSA in PBS/Tween 20 (0.05%) for 1 hr at 37° C. Serial dilutions of the antibodies are made in PBS/Tween containing 2% BSA andincubated for 1 hr at 37° C. Bound antibodies are incubated with a 1/5000 diluted peroxidase labeled...
Whey proteins are mixtures of globular proteins [β-lactoglobulin (β-Lg), α-lactalbumin (α-La), bovine serum albumin (BSA), lactoferrin (Lf), immunoglobulins (Ig)] that differ greatly in their composition and functional properties (McSweeney and Fox, 2013). Whey proteins are highly ...
(pH 7.4–7.9), 3.9 mM Tris-Cl (pH 7.4–8.0), 83.6 mM NaCl, 36.4 mM KCl, 5.1 mM MgCl2, 2 mM ATP (or ATP-γ-S), 0.2 mM Mg(CH3COO)2, 0.05% NP-40, 10% glycerol, 1 μM ZnCl2, 0.2 mM EDTA (pH 8.0), 0.4 mM EGTA, 73 μg/mL BSA, 1.2 mM imidazole, 2.1 mM DTT, ...
(SMA) as a polymer platform to prepare a micellar type of PS with two well-characterized PSs—rose bengal (RB) and methylene blue (MB)—and evaluated the outmatching benefits of SMA-PS micelles, especially focusing on the singlet oxygen (1O2) generation capacity and intracellular uptake ...
To enhance the effectiveness of bionic synovial fluid, poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCEC) hydrogel was prepared as a carrier for the bovine serum albumin (BSA) in synovial fluid. It thus significantly reduces the loss rate of BSA. Experimental results show ...
(D.1) Phagocytosis assays were performed after preincubation of macrophages for 2 h with 1 μg/mL PARylated HMGB1 or free PAR and compared with control BSA-treated macrophages. (D.2) PARP-1 protein was incubated in a poly(ADP-ribosyl)ation reaction containing NAD and activated DNA for 30...
We applied single-oocyte/embryo PAIso-seq to donated human oocytes at the germinal vesicle, metaphase I, and metaphase II stages, as well as pre-implantation embryos at the one-cell, two-cell, four-cell, eight-cell, morula, and blastocyst stages (Fig.1a). In total, we analyzed 24 oocyte...
Cells were washed with washing buffer (0.2% saponin, 3% BSA in PBS), and incubated in blocking solution (5% FCS in PBS) for 30 min. Antibody dilutions were performed in the buffer provided by the manufacturer (Biomeda Corp., Cat No. M35), and washed thrice with washing buffer. Real-...