Poly(尾-hydroxybutyrate) (PHB) production using a recombinant Escherichia coli VG1 (pTU14) was carried out. PHB granules were gradually biosynthesized in recombinant cells as inclusion bodies and the morphology of cells became linearly inflated with PHB accumulation from 6 h to 36 h of culture....
(1981) Isolation and metabolism of glycogen and poly-betahydroxybutyrate in Nocardia asteroides at different development stages. Ann. Microbiol. 132B: 13–21. CAS Google Scholar Stal, L.J., Heyer, H. & Jacobs, G. (1990) Occurrence and role of polyhydroxyalkanoate in the cyanobacterium ...
A large number of proteins/genes has been described that are necessary for and are involved in the formation of such storage PHB/PHA inclusion bodies. Detailed descriptions of key enzymes such as PHA synthases, PHA depolymerases and phasins (structural PHA granule associated proteins) are given ...
a A unique type of inclusion found in some aquatic bacteria are gas vesicles that provide flotation. Some inclusion bodies contain condensed, energy-rich organic substances, such as glycogen and poly –hydroxybutyrate (PHB). 在一些水生细菌发现的包括的一个独特的类型是提供漂浮的气体泡。 一些内含体包...
Bacillus megaterium inclusion bodies consisting of poly([R]-3-hydroxyalkanoate) (PHB) in 1926 (9). Laboratory studies showed that PHB and other polyhydroxyalkanoates (PHAs) in general are found in combination to a shortage in a non-carboneus nutrient, e.g. nitrogen. When the shortage is...
It is worth noting that all studies which stated such a stress produced by the polyester inclusion to the cell, have been performed in metabolically engineered E. coli [70-72]. In the current study a stress response of the cell while growing under CN- and N-limitation can be seen. The ...
When PhaC is overexpressed in Escherichia coli, most PhaC protein is produced as insoluble inclusion bodies due to its low aqueous solubility. This study aimed to improve the solubility of Ralstonia eutropha PHA synthase (PhaCRe) by fusing a hydrophilic tag, glutathione S-transferase (GST), to...
Graft copolymers of methyl methacrylate and poly([R]-3-hydroxybutyrate) macromonomers as candidates for inclusion in acrylic bone cement formulations: Compression testing . Journal of biomedical materials research Part B, Applied biomaterials . 2006; 77 ( 1 ):5–12. Epub 2005/10/06. doi: ...
PHAs are naturally produced polyesters stored as intracellular inclusion bodies by a wide variety of prokaryotes, particularly bacteria, in response to excess carbon under nutrient limited conditions [24]. PHAs are energy and carbon storage compounds and have been shown to provide some ecological advant...
Rigotti, D.; Dorigato, A.; Pegoretti, A. Thermo-mechanical behaviour and hydrolytic degradation of linear low density polyethylene/poly(3-hydroxybutyrate) blends.Front. Mater.2020,7, 31. [Google Scholar] [CrossRef] [Green Version] Rodríguez, L.J.; Álvarez-Láinez, M.L.; Orrego, C.E...