Download: Download full-size image Figure 2. B Cells Polarize Their MTOC toward the IS (A and B) Spleen B cells (A) or mouse lymphoma B cells (B) were incubated with anti-IgM- or anti-IgG-coated beads for 1 hr. Merged images contain the 3D projections of the α-tubulin staining. ...
Download all Video S1. Time lapse movie of the embryonic ventrolateral ectoderm during axis extension in a control (left) and a cirl null mutant (right) embryo, showing cell outlines labeled with LifeAct–mCherry (gray) and tracked cell groups as pseudo-color overlays Video S2. Time lapse...
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vinculin protein levels. Mw Molecular weight markers.cTop left: 3D cysts embedded into Matrigel/ collagen matrix of Caco-2 control (Ctr) or IRSp53-KO clones #3 and #12 cells, obtained by CRISPR/Cas9 were stained with an anti-atypical-PKC antibody (a-PKC, green), rhodamine-phalloidin to ...
Cell counts and 3D reconstructions (see below) suggest that 83E12-Gal4 is active in all larval ensheathing glial cells. However, since no 3D reconstruction has been conducted for the adult TEM volume we cannot exclude that some 83E12-Gal4 negative ensheathing glial cells exist. Fig. 1: ...
The transfected neurons expressed virtually no detectable levels of VAMP7 (Figure 3d bottom-arrow) and generated a relatively long axon-like process enriched in tau-1 protein and exhibited a similar morphology as control, ssRNA-transfected neurons (Figure 3d top). To analyze this observation ...
Unlike the spherical mutant for3Δrho3Δ [24], these cells had no obvious defect in general secretion (Figure 3D). Instead, the exocyst dispersed around the large ER-free cortex (Figures 3B, 3E, and S3D; Movies S2 and S3) where we also detected weak cortical signals of Syb1 (Figure ...
To demonstrate the live-cell imaging capability, we perform 3D-pSIMimaging of microtubules in live U2OS cells expressing α-tubulin-GFP (green fluorescent protein) (Fig.5, Supplementary Movie5). For dynamic imaging, the imaging speed should be faster than the movement of the specimen; otherwise,...