The C-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II) is a regulatory hub for transcription and RNA processing. Here, we identify PHD-finger protein 3 (PHF3) as a regulator of transcription and mRNA stability that docks onto Pol II CTD through its SPOC domain...
ANLN enhances initiated Pol II clustering and promotes Pol II CTD phase separation. Short-term depletion of ANLN alters the chromatin binding and enhancer-mediated transcriptional activity of Pol II. The target genes of ANLN-Pol II axis are involved in oxidoreductase activity, Wnt signaling and ...
Inhibition of Pol II using flavopiridol (inhibits CDK9 phosphorylation of Pol II CTD) or α-amanitin (degrades Pol II) hyper-induces sincRNAs, robustly disrupting nucleolar organization and ribosome biogenesis13. In the presence of Pol II inhibition, partly repressing the hyper-induced sincRNAs usi...
Next, we investigated which region of RNA Pol II interacts with APT. Since the CTD of RNA Pol II is a substrate of APT, it could be involved in their interaction. The CTD is essential for viability in yeast,34 and therefore, we used CRISPR-Cas9 to introduce a 3C protease cleavage site...
Pol II position in single-nucleotide resolution is then defined by mapping the 3'end of the immunoprecipitated RNA. Notably, due to the conservation of YSPTSPS repeats of Pol II CTD among eukaryotes [28], the antibody used in mNET-seq could be applicable for a broad range of species. ...
The binary interactions between MSL and PAF1C or RNF20/40 serve to stabilize their chromatin binding, which, together with H2Bub, regulate pTEFb and Pol II CTD Ser2p through a positive feedback loop. These results not only establish a function of the MSL complex in transcription regulation, ...
生化和细胞水平实验进一步揭示了INTAC中PP2A介导Pol II CTD去磷酸化并调控基因转录的机制。这项研究不仅提示了PP2A生物学功能的广谱性,也为后续研究磷酸酶和RNA核酸内切酶在转录过程中独立及协同作用的调控机制起到了很好的范式作用。 最后,这项研究也带来了新的思考,PP2A是否还存在新型的调节亚基(如其它复合物、RNA...
The splicing factors that we tested showed a significantly decreased, or trended towards a decreased, overlap with FISH spots in the DRB condition compared to DMSO, whereas MED1 and Pol II exhibited limited changes (see Methods). Extended Data Fig. 6 Phosphorylation of Pol II CTD in vitro, ...
The conserved CysA motif in the CTD of p125, previously reported to be important for the processivity ofScPol δ holoenzyme30, connects to the PIP-box and folds into a zinc finger situated next to one of the outer β-sheets of the PCNA ring (Fig.2). Consistent with these results, mut...
We characterize SPOC as a CTD reader domain that preferentially binds two phosphorylated Serine-2 marks in adjacent CTD repeats. PHF3 drives liquid-liquid phase separation of phosphorylated Pol II, colocalizes with Pol II clusters and tracks with Pol II across the length of genes. PHF3 knock-...