Safety ConsiderationRemember that you will be working with samples containing infectious virus. Follow the recommended NIH guidelines for all materials containing BSL-2 organisms. Always wear gloves, use filtered tips and work under a biosafety hood.CATALOG NUMBER: RTVSTORAGE...
MSCV based retroviral vectors carrying an IRES linkedGFP reporter gene were generated for the full length PMX1 andthe N-PMX1 fusion. Lethally irradiated mice were transplanted,two days following transduction, with post 5-FU treated BM ... K Hirose,N Pineault,RK Humphries - 《Ash Annual Meeting...
NUP98-PMX1andGFP, andmCherryorGFPonly.AClonogenic activity; Insect: Western blot detecting indicated proteins using anti-AML1 and anti-NUP98 antibodies (arrow indicates the position of NUP98-PMX1 protein
pMSCV-Cas9-2A-GFP-sgRNA 4XBS2WT-Luc IDP427 NM1-5S-tRNA-SgH PL-SIN-PGK-EGFP HA-p62 Opie2-PuroR pDL278 pcDNA3-YFP pHD7 CMV-T7-hOsa2 mCherry- SEC61B pET30a-Tn5 GFP-Rab5CA (Q79L) pAAV-EF1a-HA-hTet1CD-WPRE-PolyA pT3-EF1A-MYC-IRES-lucOS pTX179 TAL3392 GW1-Pe...
pMX-STAT5A-IRES-GFP Retroviral VectorSafety ConsiderationRemember that you will be working with samples containing infectious virus. Follow the recommended NIH guidelines for all materials containing BSL-2 organisms. Always wear gloves, use filtered tips and work under a biosafety hood.CATALOG NUMBER:...
Wnt-5a逆转录病毒载体目的构建小鼠Wnt-5a逆转录病毒载体,观察Wnt-5a在3T3细胞的表达情况.方法采用同源重组技术将小鼠Wnt-5a插入逆转录病毒载体PMX-IRES-GFP,构建PMX-IRES-Wnt5a重组质粒,利用脂质体转染3T3细胞,RT-PCR检测Wnt-5a mRNA表达情况,Western-Bloting检测Wnt-5a蛋白表达情况.结果经限制性内切酶证实成功构建了...