The pmCherry-C1 vector can be transfected into mammalian cells using any standard transfection method. If required, stable transfectants can be selected using G418 (3). pmCherry-C1 can also be used as a cotransfection marker, as the unmodified vector will express mCherry in mammalian cells. For...
pmCherry-C1 is a mammalian expression vector designed to express a protein of interest fused to the C-terminus of mCherry, a mutant fuorescent protein derived from the tetrameric Discosoma sp. red fuorescent protein, DsRed (1). The excitation and emission maxima of the native mCherry protein...
Argonaute1 fragment containing EcoRI and Bam HI site was amplified by PCR from purified tamplate from He La cell line and inserted into pmCherry-C1 fluorescent expressing vector. The recombinant pmCherry-C1-Argonaute1 plasmid was transfected into He La cells and the expression...