increasing the chances that a particular fusion will bind tightly to the amylose resin. The vectors also include a sequence coding for the recognition site of a specific protease. This allows the protein of interest to be cleaved from MBP after purification, without adding any vector-derived resid...
increasing the chances that a particular fusion will bind tightly to the amylose resin. The vectors also include a sequence coding for the recognition site of a specific protease. This allows the protein of interest to be cleaved from MBP after purification, without adding any vector-derived resid...
The system uses the pMAL vectors which are designed so that insertion interrupts a lacZα gene allowing a blue-to-white screen for inserts on X-gal. pMAL-c2 series has an exact deletion of the malE signal sequence, resulting in cytoplasmic expression of the fusion protein. pMAL-p2 series co...
pEGFP-C2载体 1. METHODS:The mouse GATA4 gene fragment was amplified by PCR from eukaryotic expression plasmid pEFSA-GATA4 and subcloned into pEGFP-C2 vector. 结果:酶切、测序结果表明将基因片段GATA4正确插入pEGFP-C2载体,并可在P19细胞中检测到GATA4基因和GATA4-EGFP融合蛋白表达。5...
pMal c X大肠杆菌表达载体说明 pMal-c2X 编号 名称 北京华越洋VECT- 70 pMal- 2X pMalc2x载体基本信息 载体名称: pMal- 2X, p Malc2X, p Mal c2X 质粒类型: 大肠杆菌蛋白表达载体 表达水平: 高 启动子: Tac 克隆方法: 多克隆位点，限制性内切酶 载体...
Figure 1: pMAL-c6T Vector The pMAL- c6T Vector has an exact deletion of the malE signal sequence. Arrows indicate the direction of transcription. Unique restriction sites in the multiple cloning site (MCS) are indicated. 产品来源 NEB-10 beta competentE. coli(pMAL-c6T) ...
increasing the chances that a particular fusion will bind tightly to the amylose resin. The vectors also include a sequence coding for the recognition site of a specific protease. This allows the protein of interest to be cleaved from MBP after purification, without adding any vector-derived resid...
The vector pMAL-p5X is designed to produce maltose-binding protein (MBP) fusions, where the protein of interest can be cleaved from MBP with the specific protease Factor Xa. MBP fusions made with this vector include an N-terminal signal sequence, so the fusion protein is directed to the per...
This allows the protein of interest to be cleaved from MBP after purification, without adding any vector-derived residues to the protein (6). For this purpose, the polylinker includes a restriction site superimposed on the sequence coding for the site of the specific protease. This is where ...
This allows the protein of interest to be cleaved from MBP after purification, without adding any vector-derived residues to the protein (6). For this purpose, the polylinker includes a restriction site superimposed on the sequence coding for the site of the specific protease. This is where ...