Indeed, we identify a novel NFAT binding site which is required for full enhancer function. We find that this site is transcriptionally active in stimulated B cells, T cells and fibroblasts and that both PMA and ionomycin are required for maximal induction. Time course analysis of the ...
Over-expression or interference of PMAIP1 in DF-1 cells after 24 h of infection with ALV-J strain SCAU-HNO6 at 105 TCID50/mL. (*P < 0.05, **P < 0.01, ***P < 0.001). Download: Download high-res image (928KB) Download: Download full-size image Figure 5. Impact of PMAIP1 ...
On the other hand, ox-LDL decreased the MMP-9 activity at ox-LDL doses of 30 to 50 µg/ml compared with full activity in PMA-activated cells. MMP-9 protein expression was then determined in cell lysates by Western blot analysis. As shown in Figure 1B and 1C, reduced MMP-9 ...
The human bladder cell lines T24 and 5637 were obtained from the Cell Bank of Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (Shanghai, China). T24 and 5637 were grown in monolayer culture at 37°C in a humidified atmosphere consisting of 5% CO2and 95% air...
As an alternative approach, fragments of an elicitor protein can be produced by digestion of a full-length elicitor protein with proteolytic enzymes like chymotrypsin or Staphylococcus proteinase A, or trypsin. Different proteolytic enzymes are likely to cleave elicitor proteins at different sites based...
Even without trypsin, there was noticeable degradation, presumably mediated by an endogenous protease, whereas incubation at a trypsin:protein ratio of 1:20 caused the 100-kDa polypeptide to disappear completely after only 0.5 min, with no trace of the 97 kDa form. Download: Download full-size ...
In the case of F-4, an initial 5-min isocratic profile at 50% B was followed by a 1-min ramp to 95% B, held there for 8 min, before a 1-min return to 50% B and re-equilibration for 4 min. Full scan spectra were obtained within m/z 100–2000 in the positive ESI mode for...