回收并纯化PCR产物,产物经1%琼脂糖凝胶电泳,在约2320bp (检测结果如图I所示)处出现特异性扩增条带,得到奶山羊的PLZF基因;然后将PCR产物、pIRES2-EGFP真核表达载体(Addgene)分别用Ecor I和BamH I双酶切后连接,将PLZF基因克隆入pIRES2_EGFP真核表达载体,构建重组载体PPLZF-IRES2-EGFP。将重组载体pPLZF-IRES2-...
张鹏, 王明秀, 敬科民, 李雨谦, 田园, 钟金城, 蔡欣.PLZF的克隆及其对犏牛未分化精原细胞的增殖作用[J]. 中国农业科学, 2024, 57(2): 390-402. ZHANG Peng, WANG MingXiu, JING KeMin, LI YuQian, TIAN Yuan, ZHONG JinChen...
This study adds new information regarding the regulation of the PLZF gene in the development and self-renewal of T cells.Du, Jiang-LongCao, XinLiu, Han YuZeng, YanYang, Xue-CaiWan, Xue-MeiChang, Fan-FanZhao, Tian-YuJia, Xiao-YeWang, Hai-ZhenLiu, JingCai, Kui-ZhengMa, Zhong-Ren...
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The development of T cells suggests that the effect of the PLZF gene on T cell differentiation and development is not cell intrinsic. However, in the neonatal mouse thymic transplant model in the Rag2/纬c-/- recipient mouse, deletion of the PLZF gene results in a significant decrease in ...
Cloning of PLZF Gene and Its Effects on the Proliferation of Undifferentiated Spermatogonia in Cattleyak 在线阅读 下载PDF 引用 收藏 分享 摘要 【目的】犏牛作为牦牛与黄牛的种间杂交产物,具有优良的生产性能,但其杂种优势的进一步应用却受限于犏牛雄性不育。通过克隆犏牛PLZF,明确其在犏牛和牦牛睾丸组织和未...
Paradoxically, differential gene expression analysis revealed that significant number of genes were upregulated upon TET2 and TET3 loss in iNKT cells. This unexpected finding could be potentially explained if loss of TET proteins was reducing the expression of proteins that suppress gene expression. In...
It is conc luded tha t PLZ F RAR /RAR PLZ F do ub le fusion gene transgenic m ice hav e hete rog ene ity o f pa thog enesis. HD AC inhibito rs such a s tr icho sta tin A, in com b ination w ith A TRA, induce d ifferen tiatio n o f the b la st/ prom y elo...
急性早幼粒白血病融合基因PML-RARaPLZF-RARa的生物学功能研究概述-’‘‘。。’__。●-。。。_。_-。_。_。_。-。一伍性呈绝迪自鱼痘(缱})的望堡堂特征是t(15;17)芹It(11;17)染色体易位,其中t(15;17)只占极少数。这种易位导致15号染色体上的PML基因或1I号染色体上的PLZF基因与17号染色体的维甲酸...
The transcription factor PLZF (promyelocytic leukemia zinc finger; zbtb16) is essential for nearly all of the unique characteristics of NKT cells including their rapid and potent response to antigen. In the immune system, zbtb16 expression is only found