A differentiation method informed by developmental biology converts human pluripotent stem cells to engraftable hematopoietic stem and progenitor cells without the use of transgenes. Adam C. Wilkinson Marella F. T. R. de Bruijn News & Views18 Sept 2024Nature Biotechnology ...
Induced pluripotent stem cells (iPSCs) can handle the low supply of cord blood and the ethical issues in embryonic stem cell research and provide a promising strategy to eliminate immune rejection. However, no complete single-cell level differentiation pathway exists for the iPSC-derived RBC ...
As such, pluripotent stem cell differentiation has been particularly useful to recapitulate the earliest events of human development allowing for the first time to study and model the NPB and its derivatives (NC and CP) in a Petri dish. The discovery of induced pluripotent stem cells (iPSCs) ...
Seeding cells on a less potent cell-binding material may minimize the inclusion of differentiated cells, exploiting the reduced adhesive properties of differentiated cells. Culturing cells with medium that supports the glycolytic pathway, using CHD7 as a biomarker for differentiation potential, and ...
Dysfunction of pancreatic δ cells contributes to the etiology of diabetes. Despite their important role, human δ cells are scarce, limiting physiological studies and drug discovery targeting δ cells. To date, no directed δ-cell differentiation method
Induced pluripotent stem cell lines from Huntington's disease mice undergo neuronal differentiation while showing alterations in the lysosomal pathway 来自 掌桥科研 喜欢 0 阅读量: 23 作者: Castiglioni,V.Onorati,M.Rochon,C.Cattaneo,E. 摘要: Huntington's disease (HD) is an autosomal dominant ...
Depending on cell types and culture conditions, such genetic alterations can lead to different metabolic phenotypes which may impact replication rates, membrane properties and cell differentiation. We here applied a comprehensive metabolomics strategy incorporating nanoelectrospray ion trap mass spectrometry (...
comprising differentiating said pluripotent stem cells in a differentiation medium consisting essentially of an effective amount of a Wnt signaling promoter in combination with an effective amount of an Activin A/Smad pathway inhibitor, optionally in combination with an effective amount of a BMP inhibitor...
Stem Cell Differentiation, Transdifferentiation, and Reprogramming Sections Figures References Abstract Background Induction of definitive endoderm Specialization into pancreatic progenitor cells Differentiation of endocrine progenitors Maturation of pancreatic β cells Concerns and recent advances in stepwise pancreat...
DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELLS TO MULTIPOTENT NEURAL CREST CELLS The present invention relates to the differentiation of human pluripotent cells, including human pluripotent stems cells to produce a self-renewing multipotent neural crest cell population in a single step method without the...