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Following the InSituPlex staining method for UltiVue panels and Akoya Opal staining protocol, FFPE samples were stained and scanned with the PhenoImager HT scanner (Akoya Biosciences). Digital assays were developed to characterize and quantify T-cell subsets, immunosuppressive cells, and cellular ...
Flow cytometry-based analysis and cell isolation were performed using the BD LSR Fortessa and BD FACS Aria III (BD Biosciences), respectively. At 24 h post transfection, B100 ml of cells were collected, centrifuged at 250g for 5 min, resuspended in PBS and analysed for expression of Cas9 (...
Figure 1: Generation of plug-and-(dis)play-mRuby cells. We aimed to make the mRuby+cell line deficient for light chain expression by deleting the VLregion in the IgK locus (Fig. 1a). In this regard, we took advantage of the facile capacity for multiplexed targeting with CRISPR-Cas9 (ref...
Plug & Play Ventures invests in companies of all sizes. They did over 250 investments in 2017 and helped them grow an average of 11%. They invest in companies they find worthy from seed round to Series C and have invested in big names such as PayPal and Dropbox. ...
Constructing a functional OMVs platform that can rapidly dis- play different tumor antigens and simultaneously display multiple tumor antigens are the keys to the development of personalized tumor vaccines. In this work, we establish a flexible tumor vaccine platform based on OMVs, using genetic ...