3.Protocol:pLKO.1puroDavideGabellini3.1MapofpLKO.1puroFig2:MapofpLKO.1containinganshRNAinsert.TheoriginalpLKO.1-TRCcloningvect..
pLKO.1.PURO 慢病毒报装Protocols Protocols > pLKO.1 Protocol Addgene is a global, non-profit plasmid repository dedicated to making it easier for scientists to share. pLKO.1 – TRC Cloning Vector Addgene Plasmid 10878. Protocol Version 1.0. December 2006. Copyright Addgene 2006, All Rights ...
Plko 1 Shctrl Puro Target Sequence 5 Cctaaggttaagtcgccctcg 3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
For a complete map of pLKO.1 containing the 1.9kb stuffer, visit www.addgene.org/10878. http://www.addgene.org/pgvec1?f=c&cmd=showcol&colid=170&page=2&pf=true(第 4/24 页)2009/2/4 12:29:07 Addgene - pLKO.1 TRC Cloning Vector Protocol Description U6 cPPT hPGK Puro R sin ...
需要金币:*** 金币(10金币=人民币1元) pLKOshRNA病毒质粒载体构建protocol方法详解.doc 关闭预览 想预览更多内容,点击免费在线预览全文 免费在线预览全文 Login | New User Search for Plasmids: Home Deposit Plasmids Request Plasmids Plasmid Tools About Addgene Information Browse Search Pricing FAQ Protocols ...
pLKOshRNA病毒质粒载体构建protocol方法详解 Seewarrantyinformationinappendix. Clickhereforaprintablecopy. TableofContents A.pLKO.1-TRCCloningVector oA.1TheRNAiConsortium oA.2MapofpLKO.1 oA.3Relatedplasmids B.DesigningshRNAOligosforpLKO.1 oB.1Determinetheoptimal21-mer ...
For a complete map of pLKO.1 containing the 1.9kb stuffer, visit www.addgene.org/10878. Description Vector Element U6 cPPT hPGK Puro R sin 3’LTR f1 ori Amp R pUC ori 5’LTR RRE Human U6 promoter drives RNA Polymerase III transcription for generation of shRNA transcripts. Central ...
Puro R Puromycin resistance gene for selection of pLKO.1 plasmid in mammalian cells. sin 3’LTR 3’ Self-inactivating long terminal repeat. f1 ori f1 bacterial origin of replication. Amp R Ampicillin resistance gene for selection of pLKO.1 plasmid in bacterial cells ...
Puro R Puromycin resistance gene for selection of pLKO.1 plasmid in mammalian cells. sin 3’LTR 3’ Self-inactivating long terminal repeat. f1 ori f1 bacterial origin of replication. Amp R Ampicillin resistance gene for selection of pLKO.1 plasmid in bacterial cells pUC ori pUC bacterial ...